Lectimers: Glycan-anchored scaffold libraries for targeting carbohydrate-binding

描述(申请人提供)：我们建议开发一种新的平台技术，该技术将产生高亲和力和高特异性的配体来靶向碳水化合物结合蛋白(CBPS)，CBPS是一种具有广泛生物学功能的蛋白质，在癌症发生、免疫调节和病毒感染中发挥作用。我们的方法建立在测序技术进步的基础上，并利用单价糖与CBPS的天然、低亲和力相互作用，以及核酸合成的简便性。因此，我们的葡聚糖锚定文库将拥有核酸的许多属性--特别是结构刚性、易于合成以及易于扩增和表征--但具有在多肽、蛋白质和小分子配体中观察到的增强的化学功能。由于这些试剂是以核酸为基础的，因此所产生的亲和剂可以相对较低的成本容易地合成，并且可以很容易地用一种 用于诊断、治疗和研究目的的各种荧光团或化学部分。

DESCRIPTION (provided by applicant): We propose to develop a novel platform technology that will produce high-affinity and high-specificity ligands to target carbohydrate binding protein (CBPs), a diverse class of proteins which possess a wide range of biological functions, playing roles cancer tumorigeneisis, immune modulation and viral infection. Our method builds upon advances in sequencing technologies and leverages the natural, low affinity, interactions of monovalent sugars for CBPs combined with the ease of synthesis of nucleic acids. Our glycan-anchored libraries will thus possess many of the attributes of nucleic acids - in particular structural rigidity, ease of synthesis and the ease with which they can be amplified and characterized - but with the enhanced chemical functionality observed in peptides, proteins and small molecule ligands. Because these reagents are based on nucleic acids, the resulting affinity agents can be readily synthesized at relatively low cost and can be easily modified with a variety of fluorophores or chemical moieties for diagnostic, therapeutic and research purposes.