Morphology & Imaging

模块使用情况和影响 形态和成像模块有两个主要功能。首先，它处理眼睛和大脑组织以进行显微镜检查。对于生理学家和分子生物学家来说，这是一项特别有价值的服务，他们没有组织学技术的经验，但对表达他们正在研究的基因或基因产物的组织和细胞进行成像非常感兴趣。组织常规处理以进行免疫细胞化学和原位杂交。吉列设计了一种用塑料植入斑马鱼胚胎的技术，这样眼睛就能稳定下来，并能以正确的方向切开。其次，该模块提供组织处理、显微镜和图像分析所使用的设备的专业知识和访问权限。该单元的另一项功能是根据要求向参与调查人员及其工作人员提供使用单元显微镜和成像设备、组织处理、原位杂交、免疫组织学技术和图像分析软件的培训。该单元还将协助缺乏必要专业知识的研究人员规划和执行免疫组织化学实验。

Module Use and Impact The Morphology and Imaging Module has two primary functions. First, it processes ocular and brain tissues for microscopy. This is a particularly valuable service for the physiologists and molecular biologists that do not have experience in histological techniques, but are very interested in imaging the tissues and cells that express genes or gene products they are investigating. Tissues are routinely processed for immunocytochemistry and in situ hybridization. Mr. Gillett devised a technique for plastic imbedding zebrafish embryos so the eyes would be stabilized and could be sectioned in the correct orientation. Second, this Module provides expertise for and access to equipment used for tissue processing, microscopy, and image analysis. An additional function of this Module is to provide training in the use of Module microscope and imaging equipment, tissue processing, in situ hybridization, immunohistological techniques, and image analysis software to participating investigators and their staff when requested. The Module will also assist with the planning and execution of immunohistochemistry experiments for investigators who lack the necessary expertise.