Morphology & Imaging

模块使用和影响 形态学和成像模块有两个主要功能。首先，它处理眼和脑组织进行显微镜检查。对于没有组织学技术经验，但对表达基因或基因产物的组织和细胞成像非常感兴趣的生理学家和分子生物学家来说，这是一项特别有价值的服务。常规处理组织用于免疫细胞化学和原位杂交。吉莱特发明了一种将塑料植入斑马鱼胚胎的技术，这样眼睛就可以稳定下来，并可以按正确的方向切片。第二，该模块提供专业知识和访问用于组织处理，显微镜和图像分析的设备。该模块的另一个功能是在需要时向参与研究者及其工作人员提供模块显微镜和成像设备、组织处理、原位杂交、免疫组织学技术和图像分析软件的使用培训。该单元还将协助缺乏必要专门知识的研究人员规划和执行免疫组织化学实验。

Module Use and Impact The Morphology and Imaging Module has two primary functions. First, it processes ocular and brain tissues for microscopy. This is a particularly valuable service for the physiologists and molecular biologists that do not have experience in histological techniques, but are very interested in imaging the tissues and cells that express genes or gene products they are investigating. Tissues are routinely processed for immunocytochemistry and in situ hybridization. Mr. Gillett devised a technique for plastic imbedding zebrafish embryos so the eyes would be stabilized and could be sectioned in the correct orientation. Second, this Module provides expertise for and access to equipment used for tissue processing, microscopy, and image analysis. An additional function of this Module is to provide training in the use of Module microscope and imaging equipment, tissue processing, in situ hybridization, immunohistological techniques, and image analysis software to participating investigators and their staff when requested. The Module will also assist with the planning and execution of immunohistochemistry experiments for investigators who lack the necessary expertise.