Nathan Shock Ctr ofExcellence in Basic Biology of Aging

描述(由申请者提供)：本申请是为续签华盛顿大学及其附属机构在衰老基础生物学方面的内森·肖克卓越中心。在过去的15年里，该中心为我们庞大的研究人员社区提供了资源，这些研究人员被资助研究衰老的基本生物学机制。我们继续在过去的资金周期中确立的科学主题上继续发展，在该主题中，比较老年学研究向我们提供了决定寿命的保守遗传途径(TOR途径就是这样的一个例子)。建议的三个研究资源核心组成部分非常适合协助这些工作：1)转基因动物模型开发；2)功能评估；3)功能基因组学。转基因动物模型开发资源核心继续将重点放在可诱导的基因表达和敲除模型上，以便能够评估遗传改变对器官和年龄的影响。功能评估核心建立在过去资助期间收集的技术资源的基础上，因此我们现在可以为研究人员提供广泛和信息丰富的生理和细胞分析，包括那些与评估小鼠模型健康寿命最相关的分析。功能基因组学核心为转录组学和蛋白质组学提供最新技术的专家协助。与我们的主题一致，它包括对翻译状态、蛋白质周转和翻译后修饰的关注。研究发展核心将继续支持衰老基础生物学方面的试验性研究项目。方案丰富核心支持行政管理、外部咨询小组、课程和研讨会方案以及数据共享和传播。

DESCRIPTION (provided by applicant): This application is for renewal of the Nathan Shock Center of Excellence in the Basic Biology of Aging at the University of Washington and affiliated institutions. This Center has over the past 15 years provided resources in support of our large community of investigators that are funded to study basic biological mechanisms of aging. We continue to build upon a scientific theme that became established in the past funding cycle, in which comparative gerontologic study informed us of conserved genetic pathways of longevity determination (the TOR pathway is an example of one such). The three proposed Research Resources Core components are well suited to assist in such efforts: 1) Transgenic Animal Model Development; 2) Functional Assessment; 3) Functional Genomics. The Transgenic Animal Model Development Resource Core continues to pursue a focus on inducible gene expression and knockout models, so that organ and age-specific effects of genetic alterations can be assessed. The Functional Assessment Core builds upon technical resources that have been gathered during the past funding period, such that we can now provide investigators with a broad and informative range of physiologic and cellular assays, including those that are most relevant to assessment of the healthspan of mouse models. The Functional Genomics Core provides expert assistance with state of the art technologies for transcriptomics and proteomics. Consistent with our theme, it includes a focus on translational state, protein turnover and post-translational modification. The Research Development Core will continue to support pilot study projects in the basic biology of aging. The Program Enrichment Core supports administrative management, an external advisory panel, a program of courses and seminars and data sharing and dissemination.