Light Microscopy Core

NHLBI光学显微镜核心设施(LMF)已经运行了13年。NHLBI-LMF由三人组成，克里斯蒂安·A·库姆斯博士(主任)、丹妮拉·A·马利德博士和吴旭峰博士(副主任)。该设施由20台显微镜组成，分布在马里兰州贝塞斯达NIH主校区10号和50号楼的几个位置。到目前为止，在过去的13年里，我们已经帮助研究人员发表了190多篇论文，并帮助几乎每个NHLBI-DIR中心和分支机构。在2011-2012年间，我们得到帮助发表了22篇论文(截至本报告之时)。在LMF中进行的研究涉及许多疾病状态、基本细胞生物学以及新成像技术的开发和实施。NHLBI-LMF的总体结构反映了NHLBI-DIR研究人员不断发展的光学显微镜需求，并承诺为NHLBI任务提供最大程度的援助。 该设施的任务是提供最先进的设备、培训和图像处理能力，以帮助NHLBI-DIR研究人员进行涉及光学显微镜的实验。在这个设施工作的研究人员可以期待核心人员提供适合他们研究的任何级别的支持。这可以包括先进的显微技术，如活体内双光子显微镜和超分辨率显微镜，到使用彩色相机进行更普通的广域成像。我们的重点是培训用户自己进行实验，尽管我们可以进行合作和各种形式的协助(实验计划、数据分析和图像处理等)。在需要的地方。 在这个设备存在的13年中，我们努力提供一种灵活且易于使用的设备，以满足研究人员的需求，并允许他们以高效的方式进行实验，即使他们之前没有显微镜经验。这些目标可以通过几种方式实现。首先，我们有一系列显微镜，可以提供广泛的显微镜技术。这些显微镜和现有的显微镜技术是根据研究所研究人员的具体需求而选择和开发的。除了显微镜，我们还在几个图像处理工作站上提供全套图像处理程序。在这些程序缺乏图像处理能力的情况下，我们要么编写自己的图像处理程序。 总而言之，该设施使用最先进的设备和专门的成像专业团队来推进NHLBI-DIR的研究任务。

The NHLBI Light Microscopy Core Facility (LMF) facility has been in operation for thirteen years. The NHLBI-LMF consists of three people, Dr. Christian A. Combs (Director), Dr. Daniela A. Malide, and Dr. Xufeng Wu (Deputy Director). The facility consists of twenty microscopes located in several locations in buildings 10 and 50 on the main NIH campus in Bethesda, MD. To date we have helped researchers publish more than 190 papers and assisted almost every NHLBI-DIR Center and Branch in the past thirteen years. In 2011-2012 we have help to publish twenty two papers (at the time of this report). Research conducted in the LMF has been on many disease states, basic cell biology, and on development and implementation of new imaging techniques. The general makeup of the NHLBI-LMF reflects the evolving light microscopy needs of NHLBI-DIR researchers and a commitment to provide a maximal level of assistance to the NHLBI mission. The mission of this facility is to provide state-of-the-art equipment, training, and image processing capabilities to assist NHLBI-DIR researchers in experiments involving light microscopy. Researchers that work in this facility can expect support from core personnel to whatever level suits their research. This can include advanced microscopy techniques like intra-vital two-photon microscopy and super-resolution microscopy to more ordinary wide-field imaging with a color camera Our emphasis is on training users to conduct the experiments themselves, although we are available for collaboration and all manner of assistance (experimental planning, data analysis and image processing, etc.) where required. Over the thirteen years this facility has been in existence we have endeavored to provide a flexible and easy to use facility that meets researchers needs and allows them to conduct their experiments in an efficient manner even if they have had no prior microscopy experience. These goals are met in several ways. First, we have an array of microscopes that offer a wide-range of microscopy techniques. These microscopes and the microscopy techniques available have been chosen and developed in response to the specific needs of researchers in the institute. In addition to the microscopes, we provide a full suite of image processing programs in several image processing workstations. Where image-processing capabilities are lacking in these programs we either write our own image processing programs. In summary this facility operates with state of the art equipment and a dedicated team of imaging professionals to further the research mission of the NHLBI-DIR.