Histology and Comparative Pathology Core

组织学和比较病理学（核心C）将为U19 CMCR资助的四个项目提供一个全面的组织病理学设施，帮助研究者进行组织学分析，以分析干细胞在改善辐射诱导胃肠道综合征（RIGS）、辐射诱导造血综合征（RIHS）和辐射诱导皮肤综合征（RICS）结局中的作用。 该中心将就样品的制备和处理以及不同研究可用的技术和程序提供咨询意见。Core将提供尸检和组织病理学服务，用于表型分析沿着全方位的组织学服务 中心提供的服务将包括：方案设计、尸检（包括组织固定）、组织石蜡块处理、冷冻包埋、标准和专用染色以及生化分析。它还将包括利用标准化分析方法对组织样本进行组织病理学评价。 核心的关键人员将是主任和组织学技术人员。主任将负责监督核心的所有活动，就不同的方法和程序提供建议，解释结果，并生成组织病理学报告和数据解释。技术人员将准备和处理适用于当前研究的组织样本，执行一些专业技术和程序（例如免疫组织化学和特殊染色）。 重要性：适当的组织样本制备（固定、处理、修剪和染色）对于本计划项目中的所有项目至关重要，因为它允许研究干细胞技术对辐射诱导损伤结果的体内影响。

The Histology and Comparative Pathology (Core C) will provide the four projects of this U19 CMCR grant a comprehensive histopathology facility that will assist the investigators in the histological analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation induce gastrointestinal syndrome (RIGS), Radiation induced hematopoietic syndrome (RIHS), and Radiation-induced cutaneous syndrome (RICS). The core will provide advice on preparation and processing of samples, as well as on the techniques and procedures available for the different studies. The Core will provide necropsy and histopathology services for phenotypic analysis along with a full range of histological services The services offered by the core will include: protocol design, necropsy, including tissue fixation, tissue proceeding to paraffin block, frozen embedding, standard and specialized stains and immunohistochemieal analyses. It will also include histopathological evaluation of tissue samples utilizing a standardized method of analysis. The key personnel of the core will be the director and the histology technical staff. The director will be responsible for supervising all the activities of the core, offering advice on the different methods and procedures, interpretation of the results, and generating histopathology reports and interpretations of the data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, perform some of the specialized techniques and procedures (e.g. immunohistochemistry and special stains). Significance: Appropriate tissue sample preparation (fixation, processing, trimming and staining) is essential to all projects in this Program Project as it allow the study of the in vivo impact of stem cell technologies on the outcome of radiation-induced injury.