ANALYTICAL METABOLIC INSTRUMENTATION CORE

这一核心是由我们以前的两个核心，即 细胞生物学和分子生物学核心。这些年来，为了应对国际社会的挑战， 我们调查人员的需求不断变化然而，2007年1月，杜守英博士，核心的全职研究 同事离开去继续她的临床培训。这为重新评估如何最好地支持 通过获取中心成员的意见，了解他们当前和未来的需求。至于细胞生物学核心， 其亚细胞-细胞器和设备分核心的仪器基地和服务一直保持着 由于不断扩大，对纯化的细胞器和膜制剂的需求大幅下降， 我们调查人员的需求不断变化这两个发展的结合为我们提供了 有机会审查和重新评估我们的调查人员对所提供的资源和服务的需求 通过这两个实体，并将其改组为一个新的、扩大的核心，即目前的名称。的 中心的外部咨询委员会和内部执行委员会提出了我们的需求， 调整的理由，并批准从赠款第3年（即2003年）开始设立目前的核心基金。 2007年3月1日）。因此，核心汇集了已经广泛和急需/使用的仪器基础， 亚细胞器和设备子核心的分析服务（多检测HPLC）菜单， 将先前的分子生物学核心转变为一个新的和扩展的中央核心（见下文的详细信息）。 此外，除了上述扩大基地提供的分析资源和服务外， 通过两个新成立的子核心增加了艺术分析和代谢资源和服务，即：一 基于质谱的蛋白质组学亚核心（Proteomic Subcore）和小分子鉴定/质谱 光谱分析子核心（小分子子核心）。中央核心的详细描述以及 这两个子核心在下面的相应部分中介绍。在此过程中，支持USC 遗传医学研究所微阵列设施，以前的分子生物学核心的子核心，是 消除，沿着与这些服务相关的中心补助金的费用。这样做的部分原因是 对这项服务的兴趣降低。然而，微阵列服务将以其他方式保持可用于 中心成员谁可能需要他们。 以下教员被任命为指导新的核心及其子核心： Murad Ookhtens博士，中央核心主任（5%的努力） 易卜拉欣·赞迪博士，基于质谱的蛋白质组学子核心主管（10%工作量） 斯坦·路易，药学博士，小分子鉴定/质谱分析子核心主管（5%工作量） 除了领导中央核心，Ookhtens博士还将与两个子核心密切协调和合作。 董事，以确保他们的服务保持响应中心的目标和需求。赞迪博士和 Louie将分别指导各自的子核心，并就其使用情况与Ookhtens博士进行沟通 报告活动。与过去一样，核心和次核心主任将被要求报告其各自的 执行委员会会议期间的表现。 除上述外。Andrew Stolz博士，前分子生物学核心主任，将继续 免费为中心服务，作为需要使用一些资源的调查人员的顾问， 这些仪器以前属于分子生物学核心。因此，他将协助Ookhtens博士 最佳利用这些资源，并确定未来必要的设备升级和购置。 中央核心和基于质谱的蛋白质组学子核心位于霍夫曼医学中心。 研究（HMR）大楼，小分子鉴定/质谱分析子核心位于隔壁 在药学院大楼里

This core resulted from the reorganization and restructuring of two of our previous cores, i.e. the Cell Biology and Molecular Biology Cores. The latter had evolved significantly over the years in response to the shifting needs of our investigators. However, in January 2007, Dr. Shouying Du, the Core's full-time Research Associate left to pursue her clinical training. This provided an opportunity to reassess how to best support the on-going and future needs of the Center members, by obtaining their input. As to the Cell Biology Core, while the instrumentation base and services of its Subcellular-Organelle and Equipment Subcore had kept expanding, the need for purified organelle and membrane preparations had declined substantially, due to shifting needs of our investigators. The combination of these two developments provided us with the opportunity to review and reassess the needs of our investigators for the resources and services offered through these two entities and to restructure them into a new and expanded core under the present title. The Center's external Advisory Board and the internal Executive Committee were presented with our needs and rationale for restructuring and approved the establishment of the present core starting with grant year 3 (i.e. 3/1/07). Thus, the core pooled the already extensive and critically needed/used instrumentation base and the menu of analytical services (multi-detection HPLC) of the Subcellular-Organelle and Equipment Subcore with that of the previous Molecular Biology Core into a new and expanded Central Core (see details below). Furthermore, to the analytical resources and services offered by the expanded base above, additional state-ofthe- art analytical and metabolic resources and services were added through two newly formed subcores, i.e.: a Mass Spectrometry-Based Proteomics Subcore (Proteomic Subcore) and a Small Molecule Identification/Mass Spectrometry Subcore (Small Molecule Subcore). The detailed descriptions of the Central Core and each of the two Subcores are presented in their respective sections below. In the process, support for the USC Institute of Genetic Medicine Microarray Facility, a Subcore of the prior Molecular Biology Core, was eliminated, along with the costs to the Center grant associated with these services. This was done in part due to the reduced interest for this service. However, microarray services will otherwise remain available to the Center Members who may need them. The following faculty were appointed to direct the new core and its subcores: Murad Ookhtens, Ph.D., Central Core Director (5% effort) Ebrahim Zandi, Ph.D., Mass Spectrometry-Based Proteomics Subcore Director (10% effort) Stan Louie, Pharm. D., Small Molecule Identification/Mass Spectrometry Subcore Director (5% effort) Besides directing the Central Core, Dr. Ookhtens will co-ordinate and work closely with the two subcore directors to insure that their services remain responsive to the Center's objectives and needs. Drs. Zandi and Louie will each direct their respective subcores and will communicate with Dr. Ookhtens regarding their usage and reporting activities. As in the past, the core and subcore directors will be required to report their respective performances during the Executive Committee meetings. In addition to the above. Dr. Andrew Stolz, Director of the previous Molecular Biology Core, will continue serving at no cost to the Center, as an advisor to investigators who need the use of some of the resources and instruments that were previously under the Molecular Biology Core. He will thus assist Dr. Ookhtens for the optimum utilization of these, as well as identify essential future equipment upgrades and acquisitions. The Central Core and the Mass Spectrometry-Based Proteomics Subcore are located in the Hoffman Medical Research (HMR) building, with the Small Molecule Identification/Mass Spectrometry Subcore located next door in the School of Pharmacy building.