Rapid,high content screening of research colonies for polymicrobial infection

描述（由申请人提供）：多微生物感染是研究界非常关注的问题，因为它们对生产力研究小组，实验结果的可靠性以及在解开基本生物学问题方面所取得的进展产生了巨大影响，这些问题是我们对人类健康问题的理解的基础。这些感染的诊断目前使用费力、昂贵和耗时的技术（例如ELISA、PCR、流式细胞术）的组合来完成。因此，例行的蜂群监测是研究的一个重大障碍。我们建议开发一个高含量的测定的基础上的物理现象的表面等离子体共振（SPR）在两种模式（光栅耦合表面等离子体共振; GCSPR和光栅耦合表面等离子体耦合发射; GCSPCE）。在该系统中采用的传感器芯片将使用两个正交的全息衍射光栅，以实现在两种模式下的同时传感器芯片询问。因此，我们预期达到高水平的灵敏度（<30 CFU/ml）和大的动态范围（5至6个对数）。由于该系统能够使用简单的流体学和廉价的芯片架构来测量来自生物样品的一千多种不同的分析物，因此该系统将能够以低劳动力需求和显著的节省来近实时地测量生物样品。该诊断系统的开发将在农业动物和人类群体的类似诊断中具有未来的相关性。我们的目标是创建一个T细胞分析系统，该系统提供高内容筛选、多参数表征能力，并采用最先进的集成样本处理以易于使用。目前，没有任何系统可以提供所提议的分析系统那样广泛的能力、简单的使用和有限的样本量要求。 公共卫生相关性：研究中的多种微生物感染可能影响殖民地健康和生产力，并可能损害实验结果的准确性。因此，旨在扩大我们对人类疾病的理解并开发更好的疗法和预防性治疗的研究处于危险之中。本申请旨在开发一种高含量测定系统，其能够以廉价且易于使用的形式立即提供对感染因子的快速、准确和灵敏的测量。该系统的使用将保护我们研究基础设施的完整性，并可能最终适用于农业牛群和人群的诊断需求。

DESCRIPTION (provided by applicant): Polymicrobial infections are of great concern to the research community because they have a dramatic impact on the productivity research groups, on the reliability of experimental results, and on the progress that can be made in unraveling fundamental biological questions that underpin our understanding of human health issues. The diagnosis of these infections is currently done using a combination of techniques (e.g. ELISA, PCR, Flow cytometry) that are laborious, costly, and time consuming. As a consequence, routine colony surveillance represents a significant impediment to research. We propose to develop a high content assay based on the physical phenomenon of surface plasmon resonance (SPR) done in two modalities (grating coupled surface plasmon resonance; GCSPR and grating coupled surface plasmon coupled emission; GCSPCE). The sensor chip employed in this system will use two orthogonal holographic diffraction gratings to enable simultaneous sensor chip interrogation in both modes. As a consequence, we anticipate reaching high levels of sensitivity (<30 CFU/ml) and a large dynamic range (5 to 6 logs). Because the system is capable of measuring more than a thousand different analytes from a biological sample using simple fluidics and an inexpensive chip architecture, this system will be capable of near real-time measurements of biological samples with a low labor demand and at dramatic savings. Development of this diagnostics system will have future relevance in similar diagnoses in agricultural animals and in human populations. The goal is to create a T cell analysis system that offers high content screening, multi-parameter characterization capability and incorporates state-of- the-art integrated sample handling for ease-of-use. At present, there are no systems available that offer the breadth of capabilities, the simplicity of use and limited sample size requirements as the proposed analysis system. PUBLIC HEALTH RELEVANCE: Polymicrobial infections in research may affect the colony health and productivity, and may compromise the accuracy of experimental results. As a consequence, research that is designed to enlarge our understanding of human disease and to develop better therapies and preventative treatments is at risk. This application aims to develop a high content assay system that is at once able to provide rapid, accurate, and sensitive measurements of infectious agents in an inexpensive and easy to use format. Use of this system will protect the integrity of our research infrastructure, and may ultimately be adaptable to diagnostic needs in agricultural herds and in the human population.