Typhoon FLA 9000 Variable Mode Imaging System

描述（由申请人提供）：美国国立卫生研究院资助的马里兰大学巴尔的摩分校的主要研究人员迫切需要一种可变模式成像系统来定量检测放射性标记、荧光标记和化学荧光分子。台风FLA 9000成像系统是一个理想的仪器，以满足我们的调查人员的需要。该仪器是生物分子成像应用的多功能主力，支持各种样品格式。这台仪器被要求更换一台老化的成像仪器，现在它已经坏了。修理这个仪器是不可行的，因为修理的费用大于仪器的价值，仪器的性能已经过时，公司不能保证将来的零件可用。台风FLA 9000仪器的最先进的能力是必要的，以满足34个NIH拨款（26个R01, 1个P01, 1个R03, 2个R21, 1个RC1, 1个R56和2个T32）授予UMB医学院19个用户的技术需求。除了该仪器外，还需要六个荧光粉屏幕和两个软件用户许可证，以优化数据收集和分析。该仪器将被安置在现有的生物化学和分子生物学仪器核心（BMBIC）。BMBIC旨在支持马里兰大学巴尔的摩校区东北象限教师的仪器需求。医学院最近投资了40万美元翻新和扩建BMBIC。该设施由BMBIC监督委员会管理，该委员会指导管理该设施的技术助理的工作。Lu-Chang博士将是目前仪器相关技术问题的主要教师资源。维护该仪器服务合同的费用将由医学院、马里兰大学格林鲍姆癌症中心和生物化学与分子生物学部门共同承担。技术助理的工资由生物化学与分子生物学系保证。所有用户的一个关键的长期研究目标是在分子水平上了解与人类疾病和癌症有关的生物事件。研究项目涵盖多个领域，包括蛋白质-蛋白质相互作用，蛋白质-核酸相互作用，DNA修复，转录调节，染色质重塑，表观遗传学，细胞信号传导，干细胞生物学和肌肉细胞生理学。由于我们老旧的磷光成像仪的故障，我们的一些研究项目遭受了严重的延误。台风FLA 9000将大大增强对放射性标记化合物的检测和定量，这对许多研究人员的实验策略至关重要，并且还将为研究人员提供一种新的基于荧光和化学荧光的检测平台，使他们能够满足美国国立卫生研究院资助的项目的需求。此外，我们认为对学生和博士后助理进行最先进的仪器培训是很重要的。新的成像系统将加强目前nih资助的五个研究生/博士后培训计划和医学博士/博士医学科学家培训计划支持的学生的培训。

DESCRIPTION (provided by applicant): NIH-funded principal investigators at the University of Maryland-Baltimore have a pressing need for a variable-mode imaging system to quantitatively detect radio labeled, fluorescently-labeled, and chemifluorescent molecules. The Typhoon FLA 9000 Imaging System is an ideal instrument to meet the needs of our investigators. This instrument is a versatile workhorse for biomolecular imaging applications that supports a wide variety of sample formats. This instrument is requested to replace an aging imaging instrument that is now broken. Repair of this instrument is not feasible, since the repair cost is greater than the instrument value, the instrument is out-dated in terms of capability, and the company cannot guarantee available of parts in the future. The state-of-the-art capability of the Typhoon FLA 9000 instrument is necessary to meet the technology needs of 34 NIH grants (26 R01, 1 P01, 1 R03, 2 R21, 1 RC1, 1 R56, and 2 T32) awarded to 19 users at the UMB School of Medicine. In addition to the instrument, six phosphor screens and two software user licenses are requested to optimize data collection and analysis. The instrument will be housed in the existing Biochemistry and Molecular Biology Instrumentation Core (BMBIC). The BMBIC is designed to support the instrumentation needs of faculty in the northeast quadrant of the University of Maryland-Baltimore campus. The School of Medicine recently invested $400,000 to renovate and expand the BMBIC. The facility is managed by a BMBIC Oversight Committee and this committee directs the efforts of a technical assistant who manages the facility. Dr. Lu-Chang will be the primary faculty resource for technical issues associated with the present instrument. Cost of maintaining the service contract for this instrument will be shared by the School of Medicine, the University of Maryland Greenebaum Cancer Center and the Department of Biochemistry and Molecular Biology. The salary of the technical assistant is guaranteed by the Department of Biochemistry and Molecular Biology. A key long range research goal of all the users is to understand biological events at the molecular level as they pertain to human disease and cancer. The research programs cover multiple areas including protein-protein interaction, protein-nucleic acid interaction, DNA repair, transcriptional regulation, chromatin remodeling, epigenetic, cell signaling, stem-cell biology, and muscle cell physiology. Due to the malfunction of our aged phosphorimager, some of our research projects have suffered serious delays. The Typhoon FLA 9000 will substantially enhance the detection and quantitation of radiolabeled compounds essential for many researchers' experimental strategies, and will also provide investigators with a new fluorescence- and chemifluorescence-based detection platform that will enable them to meet the needs of their NIH-funded programs. Additionally, we believe it is important for students and postdoctoral associates to be trained with the state-of-the-art instrumentation. The new imaging system will enhance the training of students supported by five current NIH-funded graduate/postdoctoral training programs and the MD/PhD medical scientist training program.