Stanford University Center for Reproductive and Stem Cell biology

描述(由申请人提供)：我们中心的目标是通过精心设计的人类生殖细胞发育研究，以模型系统中的知识为基础，最终应用于解决关键的临床需求，促进翻译科学领域的卓越科学。为了实现我们的目标，我们提出了三个项目、一个试点项目和四个核心。我们还提供了几个翻译试点项目的机会。这些项目是：项目I)人类胚胎干细胞和胚胎干细胞的生殖细胞分化(Reijo Pera)；项目2)男性减数分裂细胞周期的开始和发展的翻译调控(Fuller)；项目3)从原始卵泡获得成熟的人类卵母细胞(雪)；以及试点项目)卵母细胞向胚胎过渡的翻译调控(姚)。我们还提出了一个翻译试点项目，以供将来考虑治疗原发性卵巢功能不全。支持这项研究的有四个核心：A)行政；B)推广和教育；C)微观分析、排序和信息学；D)生殖数据库。这项申请是我们在过去几年中重组和规划的高潮，并展示了我们基于杰出的基础和翻译科学对生殖和干细胞生物学的愿景。这项申请是由一个合作团队提出的，该团队在基因和生殖细胞分化和成熟、途径、发育系统以及总体教育、推广和研究目标方面有着共同的兴趣。每个项目都包含一个强大的基础部分；此外，五个项目和试点中的三个项目和试点都有同样强大的临床部分，包括从多能干细胞中对人类生殖细胞发育进行遗传分析，以探索基本方面和潜在的治疗方法，获取成熟卵母细胞以补救原发卵巢功能不全，以及建立第一个POI妇女登记。每个项目都与不孕不育的女性和男性的健康有关，每个项目都由果蝇和老鼠的优雅遗传系统提供信息。我们中心的中心主题是新颖的、前瞻性的，建立在科学和临床研究的坚实基础上，具有创新的外展和教育成分，希望接触到其他科学家和医疗保健专业人员。

DESCRIPTION (provided by applicant): The objective of our Center is to promote scientific excellence in translational science via well-designed studies of human germ cell development, based on a foundation of knowledge in model systems, and culminating with applications that address critical clinical need. To accomplish our objective, we propose three projects, a pilot project, and four cores. We also present several opportunities for a translational pilot project. The projects are: Project I) Germ Cell Differentiation from Human iPSCs and hESCs (Reijo Pera); Project 2) Translational Regulation of Meiotic Cell Cycle Onset and Progression in the Male (Fuller); Project 3) Derivation of Mature Human Oocytes from Primordial Follicles (Hsueh); and Pilot Project) Regulation of Translational Control in the Oocyte to Embryo Transition (Yao). We also present a translational pilot project for future consideration on primary ovarian insufficiency. Supporting the research are four cores: A) Administration; B) Outreach and Education; C) Microanalysis, Sequencing and Informatics; D) Reproductive Database. This application is a culmination of our reorganization and planning over the last several years and presents our vision for Reproductive and Stem Cell Biology based on outstanding basic and translational science. The application is put forth by a collaborative team that shares common interests in terms of genes and germ cell differentiation and maturation, pathways, developmental systems and overall educational, outreach and research goals. Each project consists of a strong basic component; in addition, three of the five projects and pilots have an equally-strong clinical component encompassing genetic analysis of human germ cell development from pluripotent stem cells to probe fundamental aspects and potential therapies, deriving mature oocytes to remedy primary ovarian insufficiency, and establishing the first registry of women with POI. Each project is relevant to the health of infertile women and men and each is informed by the elegant genetic systems of Drosophila and the mouse. The central theme of our Center is novel, forward-looking and built on a firm foundation of scientific and clinical inquiry with an innovative outreach and educational component with hopes of reaching out to other scientists, healthcare professionals.