CORE B: STANFORD CENTER FOR REPRODUCTIVE AND STEM CELL BIOLOGY

核心 B：斯坦福生殖和干细胞生物学中心

• 批准号: 8638817
• 负责人: Renee A Reijo Pera
• 金额: $ 11.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8638817
• 关键词: Address; Area; Biochemistry; Biological Preservation; Biopsy; Blood; Budgets; California; Cell Culture Techniques; Cell Differentiation process; Cell Line; Clinical Data; Communities; Cultured Cells; DNA; Data; Development; Education; Education and Outreach; Educational Curriculum; Educational Status; Educational process of instructing; Educational workshop; Embryo; Embryology; Embryonic Development; Environment; Equipment; Faculty; Fertility; Foundations; Funding; Future; Genetic; Germ Cells; Germ Lines; Goals; Health Professional; Human Development; Image; Individual; Institutes; Institution; Karyotype determination procedure; Laboratories; Letters; Microscopy; Minority; Nurses; Ovarian Tissue; Physicians; Pilot Projects; Pluripotent Stem Cells; Postdoctoral Fellow; Publications; Recommendation; Recruitment Activity; Regenerative Medicine; Reproductive Biology; Reproductive Health; Research; Research Project Grants; Resources; Scientist; Somatic Cell; Structure; Supervision; Testis; Time; Tissue Sample; Tissues; Universities; Viral Vector; Visit; base; biobank; experience; innovation; instructor; meetings; outreach; programs; research facility; restoration; single cell analysis; square foot; stem cell biology; symposium; tissue culture

Our goal is to extend our innovative and highly-effective outreach and education program geographically, beyond the Bay Area of California, with an initiative specifically devoted to national outreach and education in Reproductive and Stem Cell Biology. We seek to accomplish this goal by leveraging one half of our current resources in terms of shared research facilities and educational programs (described below) to focus on reproductive biology and health as a central component of our general wellbeing physically, mentally and emotionally. In addition, we also include converse efforts to focus stem cell biology and regenerative medicine on reproductive biology and health as we note that the short- and long-term goals of our Center are in line with those of regenerative medicine as we address critical issues in germ cell development, embryogenesis, and fertility preservation and restoration. Objectives: The outreach and educational component of this application is built on a strong foundation, with generous financial and intellectual support from Stanford University, under the directorship of Renee Reijo Pera and co-director Margaret Fuller. This allows immediate impact, at a national level, on Reproductive and Stem Cell Biology with objectives as follows: 1. Provide physical space that is modern, well-equipped and staffed for a shared research program for individual or small group research experiences. We propose to leverage our commitment of a 2750 sq ft shared research facility, in the newly-constructed Lorry Lokey Institute for Stem Cell Biology and Regenerative Medicine, to accomplish our goals for Outreach and Education in the Stanford University Center for Reproductive and Stem Cell Biology. This shared research space is under the supervision of Dr Reijo Pera and can accommodate up to 8 visitors at any one time at all educational levels; we will dedicate 4 bench spaces for emphasis on reproductive biology. Two of these will be dedicated to occupants of 4 weeks or less; 2 will be distributed on a quarterly basis. This arrangement will allow us to host 32 visitors per year. Applications to use this space will include a brief description of research projects relevant to the goals of the SCCPRIR network and focus areas, a description of proposed benefit to be derived, a curriculum vitae and two letters of recommendation as appropriate. The shared research space provides an extraordinarily well-equipped environment with faculty oversight, as well as, lab management by Dr Vittorio Sebastiano (Instructor and former postdoctoral fellow in the Hans Scholer laboratory) with assistance from Ms Cindy Klein (a talented research associate with Drs Hsueh and Reijo Pera). This Center space incorporates research and teaching space, a modern microscopy suite, viral vector core, embryology suite, extensive tissue culture, wet lab space and our RENEW BioBank of tissue samples and clinical data from the Translational Pilot (V Baker) and other tissues collected through the years (ovarian tissue, testis biopsies, blood DNA, RNAs, embryonic and pluripotent stem cell lines for distribution). Equipment meets needs for single cell analysis, embryonic and pluripotent stem cell culture and differentiation of germ line and somatic cells, time lapse imaging, karyotyping, biochemistry and genetics. In the past 3 years, this interactive structure has contributed to outstanding publications and preliminary data for >$40M in direct funding by jump-starting research programs within the Stanford community and collaborating institutions. Here, we launch this program nationally and expect highly-competitive and innovative projects will emerge to invigorate Reproductive and Stem Cell Biology. 2. Provide a formal curriculum that incorporates laboratory-based courses and didactic symposia in Reproductive and Stem Cell Biology. We will provide dedicated courses in Reproductive and Stem Cell Biology. We propose to accomplish this, on a limited budget as mandated here, by dedicating 2 of our quarterly courses already in existence to Reproductive and Stem Cell Biology. We note that each year, we hold four laboratory-based courses on Human Development and Pluripotent Stem Cells. We will dedicate one of these courses to Reproductive and Stem Cell Biology each summer session and another in early winter (the first quarter of each year). This will allow us to recruit promising minority undergraduates (our future scientists) through SSRP (Stanford Summer Research Program Amgen Scholars), local Stanford undergraduates and those who apply to visit nationally. In addition, we note that we will designate one symposium or workshop (generally a full day) to providing formal education to the larger community of scientists, healthcare professionals and the public. Finally, we will hold our annual retreat in the Center for Reproductive and Stem Cell Biology, as we have for the last two years in January of each year and use a quarter of the available space (approximately 20-25 spaces) to include health care professionals broadly (from trainees to nurses, physicians, embryologists and assistants). We anticipate invitation of 2-5 external seminar speakers per year and more broad representation of invitees across the USA. We note that all projects and the pilot projects will equally contribute to, and receive benefit from, this core Outreach and Educational effort.

我们的目标是扩大我们的创新和高效的外展 和教育计划，超越加利福尼亚州的湾区，特别致力于一项倡议 致力于生殖和干细胞生物学方面的国家推广和教育。我们寻求通过以下方式实现这一目标 在共享研究设施和教育项目方面利用我们现有资源的一半 (如下所述)将生殖生物学和健康作为我们总体福祉的核心组成部分 无论是身体上、精神上还是情感上。此外，我们还包括聚焦干细胞的反向努力。 生物学和再生医学对生殖生物学和健康的影响正如我们所注意到的那样，短期和长期 我们中心的目标与再生医学的目标一致，因为我们解决了生殖细胞的关键问题 发育、胚胎发育以及生育能力的保存和恢复。 目标：该应用程序的外展和教育部分建立在坚实的基础上， 在Renee Reijo的指导下，斯坦福大学提供了慷慨的财政和智力支持 佩拉和联合导演玛格丽特·富勒。这使得在国家一级对生殖和健康产生直接影响 干细胞生物学的目标如下： 1.为共享的研究项目提供现代化、设备齐全和人员配备齐全的物理空间 个人或小组研究经验。我们计划利用我们2750平方英尺的承诺 共享研究设施，在新建的卡车罗基干细胞生物学和再生研究所 医学，以实现我们在斯坦福大学中心的外展和教育目标 生殖与干细胞生物学。这个共享的研究空间由Reijo Pera博士和 可以在任何时间容纳多达8名参观者，在所有教育水平；我们将专门提供4个板凳空间 强调生殖生物学。其中两个将专门用于四周或以下的居住者；两个将是 每季度分发一次。这一安排将使我们每年接待32名游客。要使用的应用程序 本栏目将简要介绍与SCCPRIR网络目标相关的研究项目 和重点领域，对拟获得的好处的说明，一份简历和两封 酌情提出建议。共享的研究空间提供了一个设备异常齐全的 由教员监督的环境以及Vittorio Sebastiano博士(讲师和 前汉斯·斯科勒实验室博士后研究员)，由Cindy Klein女士(一位才华横溢的 与薛博士和Reijo Pera博士一起研究)。这个中心空间集研究和教学于一体 空间，现代显微镜套件，病毒载体核心，胚胎学套件，广泛的组织培养，潮湿的实验室空间 和我们的更新生物库的组织样本和临床数据来自翻译试点(V Baker)和其他 多年来收集的组织(卵巢组织、睾丸活检、血液DNA、RNA、胚胎和 用于分布的多潜能干细胞系)。设备满足单细胞分析、胚胎和 多能干细胞培养和生殖系和体细胞的分化，时间推移成像，核型分析， 生物化学和遗传学。在过去的3年里，这种互动结构为我们做出了卓越的贡献 出版物和初步数据&gt；通过在全球范围内启动研究项目获得4000万美元的直接资助 斯坦福社区和合作机构。在这里，我们在全国范围内推出这一计划，并期待着极具竞争力的 并将出现创新项目，以振兴生殖和干细胞生物学。 2.提供正式课程，其中包括以实验室为基础的课程和教学研讨会 生殖与干细胞生物学。我们将提供生殖和干细胞方面的专门课程 生物学。我们建议在这里规定的有限预算内，通过将我们每个季度的两次 生殖和干细胞生物学的课程已经存在。我们注意到，每年我们都会举办四次 以实验室为基础的人类发育和多能干细胞课程。我们将把其中的一个献给 生殖和干细胞生物学课程每年夏季开课，初冬开课(第一期) 每年的季度)。这将使我们能够招收有前途的少数民族本科生(我们未来的科学家) 通过SSRP(斯坦福大学暑期研究计划安进学者)，当地的斯坦福大学本科生和 申请在全国范围内访问的。此外，我们注意到，我们将指定一个专题讨论会或讲习班。 (通常是一整天)为更大的科学家社区、医疗保健社区提供正规教育 专业人士和公众。最后，我们将在生殖和干细胞中心举行一年一度的静修 细胞生物学，因为我们在过去的两年里每年的一月和使用四分之一的可用的 空间(大约20-25个空间)，以广泛地包括卫生保健专业人员(从实习生到护士， 医生、胚胎学家和助理)。我们预计每年邀请2-5名外部研讨会演讲者 以及更广泛的受邀者在全美的代表性。 我们注意到，所有项目和试点项目都将同样对这一核心作出贡献，并从中受益。 外展和教育工作。