CORE B: STANFORD CENTER FOR REPRODUCTIVE AND STEM CELL BIOLOGY

核心 B：斯坦福生殖和干细胞生物学中心

• 批准号: 8638817
• 负责人: Renee A Reijo Pera
• 金额: $ 11.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8638817
• 关键词: Address; Area; Biochemistry; Biological Preservation; Biopsy; Blood; Budgets; California; Cell Culture Techniques; Cell Differentiation process; Cell Line; Clinical Data; Communities; Cultured Cells; DNA; Data; Development; Education; Education and Outreach; Educational Curriculum; Educational Status; Educational process of instructing; Educational workshop; Embryo; Embryology; Embryonic Development; Environment; Equipment; Faculty; Fertility; Foundations; Funding; Future; Genetic; Germ Cells; Germ Lines; Goals; Health Professional; Human Development; Image; Individual; Institutes; Institution; Karyotype determination procedure; Laboratories; Letters; Microscopy; Minority; Nurses; Ovarian Tissue; Physicians; Pilot Projects; Pluripotent Stem Cells; Postdoctoral Fellow; Publications; Recommendation; Recruitment Activity; Regenerative Medicine; Reproductive Biology; Reproductive Health; Research; Research Project Grants; Resources; Scientist; Somatic Cell; Structure; Supervision; Testis; Time; Tissue Sample; Tissues; Universities; Viral Vector; Visit; base; biobank; experience; innovation; instructor; meetings; outreach; programs; research facility; restoration; single cell analysis; square foot; stem cell biology; symposium; tissue culture

Our goal is to extend our innovative and highly-effective outreach and education program geographically, beyond the Bay Area of California, with an initiative specifically devoted to national outreach and education in Reproductive and Stem Cell Biology. We seek to accomplish this goal by leveraging one half of our current resources in terms of shared research facilities and educational programs (described below) to focus on reproductive biology and health as a central component of our general wellbeing physically, mentally and emotionally. In addition, we also include converse efforts to focus stem cell biology and regenerative medicine on reproductive biology and health as we note that the short- and long-term goals of our Center are in line with those of regenerative medicine as we address critical issues in germ cell development, embryogenesis, and fertility preservation and restoration. Objectives: The outreach and educational component of this application is built on a strong foundation, with generous financial and intellectual support from Stanford University, under the directorship of Renee Reijo Pera and co-director Margaret Fuller. This allows immediate impact, at a national level, on Reproductive and Stem Cell Biology with objectives as follows: 1. Provide physical space that is modern, well-equipped and staffed for a shared research program for individual or small group research experiences. We propose to leverage our commitment of a 2750 sq ft shared research facility, in the newly-constructed Lorry Lokey Institute for Stem Cell Biology and Regenerative Medicine, to accomplish our goals for Outreach and Education in the Stanford University Center for Reproductive and Stem Cell Biology. This shared research space is under the supervision of Dr Reijo Pera and can accommodate up to 8 visitors at any one time at all educational levels; we will dedicate 4 bench spaces for emphasis on reproductive biology. Two of these will be dedicated to occupants of 4 weeks or less; 2 will be distributed on a quarterly basis. This arrangement will allow us to host 32 visitors per year. Applications to use this space will include a brief description of research projects relevant to the goals of the SCCPRIR network and focus areas, a description of proposed benefit to be derived, a curriculum vitae and two letters of recommendation as appropriate. The shared research space provides an extraordinarily well-equipped environment with faculty oversight, as well as, lab management by Dr Vittorio Sebastiano (Instructor and former postdoctoral fellow in the Hans Scholer laboratory) with assistance from Ms Cindy Klein (a talented research associate with Drs Hsueh and Reijo Pera). This Center space incorporates research and teaching space, a modern microscopy suite, viral vector core, embryology suite, extensive tissue culture, wet lab space and our RENEW BioBank of tissue samples and clinical data from the Translational Pilot (V Baker) and other tissues collected through the years (ovarian tissue, testis biopsies, blood DNA, RNAs, embryonic and pluripotent stem cell lines for distribution). Equipment meets needs for single cell analysis, embryonic and pluripotent stem cell culture and differentiation of germ line and somatic cells, time lapse imaging, karyotyping, biochemistry and genetics. In the past 3 years, this interactive structure has contributed to outstanding publications and preliminary data for >$40M in direct funding by jump-starting research programs within the Stanford community and collaborating institutions. Here, we launch this program nationally and expect highly-competitive and innovative projects will emerge to invigorate Reproductive and Stem Cell Biology. 2. Provide a formal curriculum that incorporates laboratory-based courses and didactic symposia in Reproductive and Stem Cell Biology. We will provide dedicated courses in Reproductive and Stem Cell Biology. We propose to accomplish this, on a limited budget as mandated here, by dedicating 2 of our quarterly courses already in existence to Reproductive and Stem Cell Biology. We note that each year, we hold four laboratory-based courses on Human Development and Pluripotent Stem Cells. We will dedicate one of these courses to Reproductive and Stem Cell Biology each summer session and another in early winter (the first quarter of each year). This will allow us to recruit promising minority undergraduates (our future scientists) through SSRP (Stanford Summer Research Program Amgen Scholars), local Stanford undergraduates and those who apply to visit nationally. In addition, we note that we will designate one symposium or workshop (generally a full day) to providing formal education to the larger community of scientists, healthcare professionals and the public. Finally, we will hold our annual retreat in the Center for Reproductive and Stem Cell Biology, as we have for the last two years in January of each year and use a quarter of the available space (approximately 20-25 spaces) to include health care professionals broadly (from trainees to nurses, physicians, embryologists and assistants). We anticipate invitation of 2-5 external seminar speakers per year and more broad representation of invitees across the USA. We note that all projects and the pilot projects will equally contribute to, and receive benefit from, this core Outreach and Educational effort.

我们的目标是扩大我们的创新和高效的推广， 在地理上，在加州湾区之外， 生殖和干细胞生物学的国家推广和教育。我们力求实现这一目标， 在共享研究设施和教育项目方面利用我们现有资源的一半 （下文所述）将生殖生物学和健康作为我们总体福祉的核心组成部分 身体上，精神上和情感上此外，我们还包括逆向努力，重点干细胞 生物学和再生医学对生殖生物学和健康的影响，因为我们注意到， 我们中心的目标与再生医学的目标是一致的，因为我们解决生殖细胞的关键问题 发育、胚胎发生以及生育力的保存和恢复。 目标：该应用程序的推广和教育部分建立在坚实的基础上， 在勒尼·雷霍的领导下，斯坦福大学慷慨的财政和智力支持 导演Margaret Fuller这就能在国家一级对生殖健康和生殖健康产生直接影响， 干细胞生物学的目标如下： 1.为共享研究计划提供现代化、设备齐全和人员配备的物理空间， 个人或小组研究经验。我们建议利用我们承诺的2750平方英尺 共享研究设施，在新建成的劳里洛基研究所干细胞生物学和再生 医学，以实现我们在斯坦福大学中心的推广和教育目标， 生殖与干细胞生物学这个共享的研究空间是在Reijo Pera博士的监督下， 可以容纳多达8名游客在任何一个时间在所有教育水平;我们将致力于4个长凳空间， 强调生殖生物学。其中两个将专门用于居住4周或更短时间; 2个将是 按季度发放。这一安排将使我们每年接待32名游客。应用程序使用 这一部分将简要介绍与SCCPRIR网络目标相关的研究项目 和重点领域、对拟议收益的描述、简历和两封信 酌情推荐。共享的研究空间提供了一个装备精良的 环境与教师的监督，以及，实验室管理博士维托里奥Pittiano（讲师和 前汉斯·肖勒实验室博士后研究员），并得到辛迪·克莱因女士（一位才华横溢的 研究助理与博士薛和Reijo Pera）。这个中心空间结合了研究和教学 空间，现代显微镜套件，病毒载体核心，胚胎学套件，广泛的组织培养，湿实验室空间 和我们的RENEW生物库的组织样本和临床数据从翻译试点（V贝克）和其他 多年来收集的组织（卵巢组织，睾丸活检，血液DNA，RNA，胚胎和 用于分布的多能干细胞系）。设备满足单细胞分析、胚胎和 多能干细胞培养和生殖系和体细胞的分化，延时成像，核型分析， 生物化学和遗传学。在过去的3年里，这种互动结构为杰出的 出版物和初步数据>$40M的直接资助，由启动研究计划内， 斯坦福大学社区和合作机构。在这里，我们在全国范围内推出这一计划，并期望高度竞争 和创新项目将出现振兴生殖和干细胞生物学。 2.提供一个正式的课程，包括实验室为基础的课程和教学研讨会， 生殖与干细胞生物学我们将提供生殖和干细胞方面的专门课程 生物学我们建议在这里规定的有限预算内，通过将我们的季度 生殖和干细胞生物学课程已经存在。我们注意到，我们每年举行四次 以实验室为基础的人类发育和多能干细胞课程。我们将把其中一个 课程生殖和干细胞生物学每年夏季会议和另一个在初冬（第一次 每年的四分之一）。这将使我们能够招募有前途的少数民族本科生（我们未来的科学家） 通过SSRP（斯坦福大学暑期研究计划安进学者），当地斯坦福大学本科生和 申请出国访问的人员。此外，我们注意到，我们将指定一个专题讨论会或讲习班， （通常是一整天），为更大的科学家社区提供正规教育，医疗保健 专业人士和公众。最后，我们将在生殖和干细胞中心举行年度务虚会， 细胞生物学，因为我们在过去的两年中，每年一月，并使用四分之一的可用 空间（大约20 - 25个空间），以广泛包括卫生保健专业人员（从受训人员到护士， 医生、胚胎学家和助理）。我们预计每年邀请2 - 5名外部研讨会发言人 以及美国各地更广泛的受邀者代表性。 我们注意到，所有项目和试点项目都将同样有助于这一核心，并从中受益。 外展和教育工作。