Microengineered Osteons for Bone Tissue Engineering

描述（由申请人提供）： 骨组织工程中的微工程化骨单位承重骨缺损的修复是临床上的一个重要问题。通常，骨移植物需要用于重建骨骼手术，以帮助骨折愈合、骨融合、植入物整合和骨骼缺损的修复，但是同种异体骨的使用受到高失败率的限制。组织工程构建物是有前途的替代物。然而，用于骨修复的工程化组织通常受到缺乏血管、难以工程化复杂的骨微结构和支架机械强度不足的限制。我们建议使用多尺度的方法，结合纳米级分子排列，微工程组织和仿生骨支架骨组织工程。因此，我们的目标是产生微工程骨样单位和仿生支架内整合所得的结构，并在体外和体内分析的结构。这将导致矿化血管组织在各种功能和生理相关尺度上具有受控的结构。预计该项目将通过在皮质骨内形成复杂的微结构（如具有血管通道的骨样单位），在骨组织工程中产生新的范例。 公共卫生相关性： 承重骨缺损的修复仍然是一个重大的临床挑战。该项目有望通过在皮质骨内形成具有血管通道的骨样单位等复杂微结构，在骨组织工程中产生新的范例。

DESCRIPTION (provided by applicant): Microengineered osteons for bone tissue engineering Repair of load bearing bone defects remains a significant clinical problem. Often bone grafts are required for reconstructive skeletal procedures to aid fracture healing, bone fusion, implant integration and repair of skeletal defects, but the use of allograft bone is limited by a high failure rate. Tissue engineered constructs are promising substitutes. However, engineered tissues for bone repair are often limited by a lack of vascularity, the difficulty to engineer the complex bone microarchitecture and insufficient scaffold mechanical strength. We propose to use a multi-scale approach to bone tissue engineering that combines nanoscale molecular alignment, microengineered tissues and biomimetic bone scaffolds. We therefore aim to generate microengineered osteon-like units and to integrate the resulting structures within biomimetic scaffolds and to analyze the constructs in vitro and in vivo. This will result in mineralized vascular tissues with controlled architectures on a variety of functional and physiologically relevant scales. It is expected that this project will generate a new paradigm in bone tissue engineering by enabling the formation of complex microstructures such as osteon-like units with vascular channels within cortical bone. PUBLIC HEALTH RELEVANCE: Repair of load bearing bone defects remains a significant clinical challenge. This project is expected to generate a new paradigm in bone tissue engineering by enabling the formation of complex microstructures such as osteon-like units with vascular channels within cortical bone.