Bioengineering Research Partnership in Total Joint Replacements

项目摘要 本提案是对题为"生物工程研究"的项目公告（PAR-07 - 352）的回应 伙伴关系"。在这个大学-产业合作伙伴关系中，我们专注于开发和测试 金刚石对金刚石和金刚石对聚乙烯表面，用于最大限度地减少关节之间的磨损 骨科器械中的组件。磨损引起的并发症包括部件松动， 有害的生物反应，骨质溶解，机械不稳定，关节活动度降低，疼痛增加， 最终导致植入失败。在本项目中，UAB和Smith & Nephew，Inc.团队开发和测试 金属（CoCrMo或Ti-6Al-4V）骨科器械上独特的纳米结构多层金刚石涂层， 减少关节部件的摩擦和磨损。我们建议进行一项为期五年的研究， 具体目标1：开发纳米技术，以减少摩擦和磨损， 髋关节和膝关节金属植入物的关节、承重部件， 具有交替纳米和微米结构分层的多层结构的金刚石涂层， 件.具体目标2：证明多层金刚石涂层（用于金刚石对金刚石） 金刚石或聚乙烯上的金刚石关节）与单 层金刚石对金刚石接合或金属对聚乙烯或金属对金属接合 用于商业骨科器械。具体目标3：进行涉及金刚石的磨损模拟器研究 使用行业标准多轴髋关节和膝关节模拟器的涂层髋关节和膝关节部件 位于Smith & Nephew，Inc.具体目标4：评价细胞对金刚石磨屑颗粒的反应， 与聚乙烯和钴Chrome碎屑颗粒相比。具体目标5：进行体内研究 表征对照和金刚石生物材料的初始短期器官和细胞反应， 总体生物相容性特征。将植入（注入）从摩擦学研究中收集的磨损碎屑 进入成年大鼠的滑膜样气囊模型短期（数小时至数天）以评估临床， 细胞、组织学和细胞因子生物相容性特征。椎间盘植入骨小梁区域 家兔将评估初始组织液和血液与对照和涂层植入物表面的相互作用， 从几个小时到几个月不等。该拟议BRP的总体临床影响将改善 将整个关节置换的使用寿命延长至30年以上， 需要反复多次外科手术。

Project Summary This proposal is in response to program announcement (PAR-07-352) entitled "Bioengineering Research Partnerships (BRP)". In this University-Industry Partnership, we are focused on development and testing of diamond-on-diamond and diamond-on-polyethylene surfaces for minimizing wear between articulation components in orthopaedic devices. Complications arising from wear include component loosening, deleterious biological responses, osteolysis, mechanical instability, decreased joint mobility, increased pain, and ultimately implant failure. In this project, UAB and Smith & Nephew, Inc. team up to develop and test unique nanostructured multilayer diamond coatings on metal (CoCrMo or Ti-6Al-4V) orthopaedic devices with the goal of reducing friction and wear in articulation components. We propose a five-year study to focus on the following specific aims: Specific Aim 1: Develop nanotechnology for reducing friction and wear on the articulating, load-bearing components of hip and knee metallic implants by chemical vapor deposition (CVD) of a diamond coating having a multilayer structure with alternating nano- and micro-structural layered components. Specific Aim 2: Demonstrate that the multilayer diamond coatings (intended for diamond-on- diamond or diamond-on-polyethylene articulation) exhibit improved wear resistance when compared to single- layer diamond-on-diamond articulation or to the metal-on-polyethylene or metal-on-metal couples currently used in commercial orthopaedic devices. Specific Aim 3: Perform wear simulator studies involving diamond coated hip and knee articulation components using the industry-standard multi-axis hip and knee simulators located at Smith & Nephew, Inc. Specific Aim 4: Evaluate cellular response to diamond wear debris particles, as compared to polyethylene and cobalt chrome debris particles. Specific Aim 5: Perform in vivo investigations to characterize the initial short term organic and cellular responses of control and diamond biomaterials to overall biocompatibility profiles. Wear debris collected from the tribological studies will be implanted (injected) into the synovial-like air pouch model in the adult rat for short term (hours to days) in order to assess clinical, cellular, histological and cytokine biocompatibility profiles. Implants of discs into trabecular bone regions of rabbits will assess initial tissue fluid and blood interactions with control and coated implant surfaces for time points ranging from hours to months. The overall clinical impact of this proposed BRP would be in improving the service life time of total joint replacements to more than thirty years and hence dramatically reducing the need for recurrent multiple surgical procedures.