TRANSGENIC MOUSE AND ANIMAL MANAGEMENT

动物管理和转基因小鼠核心的目标是提供转基因和指定的 基因敲除的动物被培育到不同的遗传背景中，用于本计划的三个项目中的每一个项目 项目由于每个项目都需要大量的鼠标，并且由于一些 基因型在多个项目中共享，以确保最佳的质量控制， 动物的识别和顺畅流动，最好完成动物的育种和基因分型功能 在一个核心内由一个致力于该功能的团队进行。此外，订购、接收、进入数据库， 来自NIA菌落的老化和年轻Fischer 344的分配将由Core C管理。核心 该团队将由施方货车博士领导，并由研究所的托马斯马丁博士共同领导。 比较医学货车博士在转基因动物的育种和基因分型方面有着丰富的经验。 小鼠核心C中的机制有助于小鼠繁殖、断奶、纹身、尾部活检和 Southern印迹/PCR确定基因型。多项质量控制措施到位，以确保 在规定的时间间隔，通过Southern、北方、Western印迹法对每个品系的代表性小鼠进行品系鉴定， 沿着所示的活性测定。Martin博士在小鼠的护理和使用方面具有丰富的经验， 设计了一个全面的描述护理和管理老年小鼠在我们的殖民地。芯C 将在五年的计划项目期间为三个项目中的每一个项目提供服务。

The goal of the Animal Management and Transgenic Mouse Core is to provide transgenic and the indicated knockout animals bred into distinct genetic backgrounds to each of the three projects of this Program Project. Due to the extensive numbers of mice that are required for each project, and since some of the genotypes are shared across more than one project, in order to ensure optimal quality control, mouse identification and smooth flow of animals, the functions of breeding and genotyping of animals are best done within a core by a team dedicated to that function. Further, the ordering, receipt, entry into the database, and distribution of aged and young Fischer 344 from the NIA colonies will be managed by Core C. In Core C, this team will be led by Dr. Shi Fang Van, and co-lead by Dr. Thomas Martin of the Institute of Comparative Medicine. Dr. Van has extensive experience in the breeding and genotyping of transgenic mice. Mechanisms are in place in Core C to facilitate mouse breeding, weaning, tattooing, tail biopsy and Southern blotting/PCR to determine genotype. Multiple quality controls are in place to ensure consistency of the strains by Southern, Northern, Western blotting of representative mice per strain at specified intervals, along with activity assays as indicated. Dr. Martin has extensive experience in the care and use of mice and has designed a thorough description of the care and management of aged mouse in our colonies. Core C will serve each of the three projects during each of the five years of the Program Project.