Novel platform for isolation of rabbit monoclonal antibodies against modified RNA bases

描述（由申请人提供）：RNA编辑和转录后修饰正在成为微调遗传多样性和RNA功能的关键表观遗传机制，在大脑中特别重要。然而，虽然一些RNA编辑过程已经得到了相对较好的描述，但其他RNA修饰却知之甚少，而且关于这些过程对健康和疾病中神经功能的真正意义仍然存在许多问题。深度测序方法的最新发展突出了RNA碱基修饰的数量和程度，但需要具有必要的高特异性的工具来理解这些变化在发育，区域，细胞和亚细胞水平上在神经生理学中的作用。在这一概念验证I期研究中，我们建议使用我们的尖端技术，该技术将兔免疫系统的强大特性与酵母展示技术的能力相结合，以证明这种方法的能力，以分离出针对腺苷、肌苷、N6-甲基腺苷和N1-甲基腺苷三种衍生物的高特异性、高亲和力兔单克隆抗体。

DESCRIPTION (provided by applicant): RNA editing and post-transcriptional modification is emerging as a key epigenetic mechanism for fine tuning genetic diversity and RNA function of particular importance in the brain. However, while some RNA editing processes have been relatively well described, other RNA modifications are poorly understood and many questions remain about the true significance of these processes to neurological function in health and disease. Recent developments in deep sequencing approaches have highlighted the number and extent of RNA base modifications, but tools with the requisite high specificity are required to understand the role these changes play in neurophysiology at the developmental, regional, cellular and subcellular level. In this proof of concept Phase I study, we propose to use our cutting edge technology that combines the robust nature of the rabbit immune system with the power of yeast display technology to demonstrate the power of this approach to isolate highly specific, high affinity rabbit monoclonal antibodies against three derivatives of adenosine, inosine, N6-methyl-adenosine and N1-methyl-adenosine.