XFe96 Analyzer

描述(由申请者提供)：范德比尔特大学正在为细胞外通量分析仪申请资金。医学院和工程学院的教职员工将使用这种仪器。范德比尔特没有分析仪或类似的仪器。该分析仪允许对贴壁或悬浮细胞、渗透细胞、分离的线粒体或整个生物体中的细胞生物能量学进行非侵入性实时研究。为了提高数据的再现性，还需要一个配套的预备站，其中包括一个集成的介质交换器和非二氧化碳培养箱。参与研究的研究人员研究了许多与代谢功能障碍有关的疾病状态，如癌症、肥胖和神经退行性疾病。为了便利他们由小灵通资助的研究，必须具备在各种生物样本中轻松评估能源需求的能力。 分析仪是一个被广泛接受的新陈代谢研究平台。有七个主要用户和三个次要用户。该分析仪将由范德比尔特高通量筛选(VHTS)设施进行维护和管理。VHTS在基于平板的、多条件的生物现象讯问方面拥有专业知识。VHTS是一个非常重要且非常成功的设施，由Vanderbilt提供财政支持进行维护。总而言之，该分析仪将极大地加强范德比尔特生物能量学目前和未来的研究。

DESCRIPTION (provided by applicant): Vanderbilt University is requesting funds for an Extracellular Flux Analyzer. Faculty in the School of Medicine and in the School of Engineering will use this instrument. Vanderbilt does not possess an Analyzer or equivalent instrumentation. The Analyzer allows the non-invasive real-time study of cellular bioenergetics in adherent or suspension cells, permeabilized cells, isolated mitochondria or whole organisms. To improve data reproducibility a companion Prep Station that contains an integrated media exchanger and non-CO2 incubator is also requested. Participating investigators study numerous disease states in which metabolic dysfunction has been implicated such as cancer, obesity and neurodegenerative disorders. In order to facilitate their PHS-funded studies it is essential that the ability to easily evaluate energy requirements in a variety of biological samples be available. The Analyzer is a widely accepted platform for the study of metabolism. There are seven major users and three minor users. The Analyzer will be maintained and administered by the Vanderbilt High Throughput Screening (VHTS) facility. The VHTS has expertise in plate-based, multi-condition interrogation of biological phenomena. The VHTS is an essential and highly successful facility and is maintained by financial support from Vanderbilt. In summary, the Analyzer will dramatically enhance current and future research in bioenergetics at Vanderbilt.