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Implantable biosensors for real-time in vivo interrogation of biological phenomena

用于实时体内生物现象询问的植入式生物传感器

基本信息

批准号：
1159344
负责人：
Gary Sayler
金额：
$ 30万
依托单位：
University of Tennessee Knoxville
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-15 至 2016-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1159344&HistoricalAwards=false
关键词：
Implantable biosensors real time vivo

项目摘要

Sayler1159344This research effort is focused on the merging of light-emitting (bioluminescent) human cell lines with integrated circuit microluminometers to create miniaturized, implantable biosensing interfaces for internal monitoring of animal physiology. Conventional imaging technology relies on external cameras to penetrate and identify light-emitting cellular signatures embedded within small animal subjects. Unfortunately, due to absorption, attenuation, and scattering, even the most sensitive ultracooled cameras are unable to detect light signals beyond depths of a few centimeters in living tissue. Without major advances in imaging hardware sensitivity and/or light signal emission strength of engineered implanted cells, bioimaging applications that promise real-time, noninvasive visualization of health status in small animals may never achieve the transformational leap towards imaging, tracking, and diagnosing diseases in larger animals (i.e., humans). It is hypothesized that it may be more feasible and practical to image internally rather than externally, and that the bioengineering of an implantable optical chip capable of detecting light from engineered human cells tuned to human disease states may be a solution for effective whole-body human imaging. The intellectual merit of this research is a transformative new imaging technology that brings us closer to biosensing strategies evolved towards autonomous sense-and-respond human biotherapies where disease states are automatically recognized and treated, the outcome of which permits real-time and remote patient to doctor relationships and transformations in personalized medicine and disease care management at greatly reduced costs. Broader impacts include postdoctoral training and integration of undergraduate students within the research project, multidisciplinary collaborations between the life sciences and engineering disciplines, broad dissemination of research results, and outreach efforts centered on K-12 Science, Technology, Engineering & Mathematics (STEM) initiatives.

Sayler 1159344这项研究工作的重点是将发光（生物发光）的人类细胞系与集成电路微光度计相结合，以创建小型化、可植入的生物传感接口，用于动物生理学的内部监测。传统的成像技术依赖于外部相机来穿透和识别嵌入小动物受试者体内的发光细胞特征。不幸的是，由于吸收、衰减和散射，即使是最灵敏的超冷相机也无法检测到活组织中超过几厘米深度的光信号。在成像硬件灵敏度和/或工程植入细胞的光信号发射强度方面没有重大进展的情况下，承诺对小动物的健康状态进行实时、非侵入性可视化的生物成像应用可能永远不会实现向较大动物（即，人类）。据推测，它可能是更可行和实用的图像内部，而不是外部，和生物工程的植入式光学芯片能够检测光从工程人类细胞调谐到人类疾病状态可能是一个解决方案，有效的全身人体成像。这项研究的智力价值是一种变革性的新成像技术，它使我们更接近生物传感策略，这些策略朝着自动感知和响应人类生物疗法发展，其中疾病状态被自动识别和治疗，其结果允许实时和远程患者与医生的关系以及个性化医疗和疾病护理管理的转变，成本大大降低。更广泛的影响包括博士后培训和本科生在研究项目中的整合，生命科学和工程学科之间的多学科合作，研究成果的广泛传播，以及以K-12科学，技术，工程数学（STEM）计划为中心的外展工作。