Human Embryonic Stem Cell Core laboratory

核心A提供了成功完成本计划中每个项目所必需的专业知识和资源。核心A被安置在埃里森干细胞核心（ESCC），一个专门设计和建造的设施，提供组织培养罩，一些蔡司显微镜，活细胞成像能力，和缺氧准备孵化器。此外，Core A还提供了用于培养和维持人类多能干细胞的经验证试剂，最大限度地减少了不同供应商或批次之间可能发生的实验变异性。核心A还教导和维护一个“最佳实践”环境，最大限度地减少可能导致污染和培养漂移的偏差。然而，也许最伟大的 重要的是由POI调查员在ESCC内的共同定位产生的优势。通过聚集来自不同学科的研究人员，ESCC聚集了专业知识。这使得来自一个实验室的研究人员能够立即从其他实验室的进展中受益，促进了多能系之间重要生物学差异的鉴定，并促进了受益于多个群体贡献的合作努力;最近的一个例子是采用无载体方法进行诱导多能干细胞（iPSC）生成。 核心A将通过提供核心服务、将其知识和资源提供给调查机构调查人员使用以及发展新的知识和能力，促进实现整个调查机构的目标。在目前的资助期间，核心A为进一步加深我们对干细胞生物学的了解做出了重要贡献。本申请提出通过表征新一代“幼稚样”人多能干细胞系，通过开发将增强人多能干细胞系产生感兴趣组织的可预测性的培养条件，以及通过寻求鉴定多能性和定向分化的代谢驱动因素，继续这种思路。

Core A provides expertise and resources that are essential for successful completion of each of the Projects within this Program. Core A is housed in the Ellison Stem Cell Core (ESCC), a purpose-designed and built facility providing access to tissue culture hoods, a number of Zeiss microscopes, live cell imaging capability, and hypoxia-ready incubators. Furthermore, Core A provides access to validated reagents for the culture and maintenance of human pluripotent stem cells, minimizing experimental variability that can occur between different vendors or batches. Core A also teaches and maintains a "best practices" environment that minimizes deviations that can lead to contamination and culture drift. However, perhaps of greatest importance are the advantages that result from the co-localization of POI investigators within the ESCC. By aggregating researchers from different disciplines, the ESCC aggregates expertise. This allows researchers from one laboratory to immediately benefit from advances made by other laboratories, facilitates the identification of important biological differences between pluripotent lines, and fosters collaborative efforts that benefit from contributions from multiple groups; a recent example being the adoption of vector-free methods for induced pluripotent stem cell (iPSC) generation. Core A will facilitate the goals of the overall POI through the provision of core services, by placing its knowledge and resources at the disposal of POI investigators, and by developing new knowledge and capabilities. During the current funding period Core A has made important contributions toward furthering our knowledge of stem cell biology. The current application proposes to continue in this vein by characterizing a new generation of "naive-like" human pluripotent stem cell lines, by developing culture conditions that will enhance the predictability with which human pluripotent stem cell lines generate tissues of interest, and by seeking to identify metabolic drivers of pluripotency and directed differentiation.