Integrated Technologies for Polymeric Biomaterials, RESBIO

描述（由申请人提供）：在本次更新中，RESBIO生物医学技术资源中心将以其在开发和应用先进方法发现和优化生物材料方面的开创性成就为基础。第三个资助期的目标是a)扩大对细胞-材料相互作用中涉及的基本信号机制的理解，b)推进组织支架制造的新方法和新技术，以及c)测试这些进展在干细胞研究、组织工程和再生医学中的应用。六项高度协调的技术研究和开发项目将由一个跨越五所大学和一个国家实验室的团队进行。在Project 1中，化学家和材料科学家将制备具有整个体积表达的配体的水凝胶和具有表面系留配体的纳米纤维垫，以促进特定生物相互作用的研究。他们还将构建可在其他核心项目、合作者和生物医学社区中分发的已定义的测试平台。在项目2中，生物学家将开发支持干细胞自我更新的复合生物合成材料。项目3将开发方法来研究细胞和生物材料之间的机械相互作用如何调节细胞功能，从而为生物活性支架的设计和制造提供信息。项目4团队将推进基于成像的方法，研究生物材料驱动的核和细胞骨架探针组织，用于表征干细胞细胞内信号传导和3D环境中的组织行为。项目5的目标是开发一种无创功能性拉曼成像工具，以微尺度分辨率生成活细胞和组织的3D化学分辨率图像。项目6将扩展其多尺度计算分子建模方法，以指导项目1和项目2中生物活性聚合物设计的优化。合作将通过干细胞表征项目挑战RESBIO的新兴技术；骨、软组织和神经再生；血管生成生物材料的发现；以及蛋白质和聚合物的相互作用。已建立的服务、培训和传播计划将扩大核心项目的新内容，并提供给更广泛的科学界。

DESCRIPTION (provided by applicant): In this renewal, the RESBIO Biomedical Technology Resource Center will build on its pioneering achievements in the development and application of advanced methods for discovery and optimization of biomaterials. The goals of the third funding period are a) expanding understanding of basic signaling mechanisms involved in cell-materials interactions, b) advancing new methods and technologies for creation of tissue scaffolds, and c) testing these advances in applications for stem cell research, tissue engineering and regenerative medicine. Six highly coordinated Technology Research and Development Projects will be conducted by a team spanning five universities and one national laboratory. In Project 1, chemists and material scientists will prepare hydrogels with ligands expressed throughout the volume and nanofiber mats with surface-tethered ligands to facilitate the study of specific biological interactions. They will also fabricate defined test beds that can be distributed among other Core projects, collaborators, and the biomedical community. In Project 2, biologists will develop composite biologic-synthetic materials that support stem cell self-renewal. Project 3 will develop approaches to enable the study of how mechanical interactions between cells and biomaterials regulate cell function and thus inform the design and fabrication of bioactive scaffolds. The Project 4 team will advance imaging-based methods to study biomaterial-driven organization of nuclear and cytoskeletal probes used to characterize stem cell intracellular signaling and organizational behaviors in 3D environments. Project 5's goal is to develop a noninvasive functional Raman imaging tool to produce 3D, chemically resolved images of live cells and tissues at microscale resolution. Project 6 will extend its multiscale computational molecular modeling approaches to guide optimization of the bioactive polymer design in Projects 1 and 2. Collaborations will challenge RESBIO's emerging technologies with projects on stem cell characterization; bone, soft tissue, and nerve regeneration; discovery of angiogenic biomaterials; and protein-polymer interactions. Well-established programs of Service, Training and Dissemination will be expanded with new content from the Core Projects, and delivered to a wider scientific community.