Leica SP8 WWL Confocal Microscope

描述（由申请人提供）：我们正在申请购买Leica SP 8 WLL共聚焦显微镜的资金。该仪器将被放置在华盛顿大学的凯克成像设施。凯克设施是一个完善的，非常频繁使用的设施，提供共聚焦显微镜仪器20年，服务于50多个华盛顿大学研究实验室在2012年。该设施现在有两个过时和磨损的共焦显微镜，其中一个不再由其制造商支持。所要求的仪器需要为我们的50名用户提供显微镜访问，并为6名主要用户提供必要的扩展技术能力，以推进从单细胞内的动态到整个胚胎发育的研究项目。因此，建议的仪器将需要分析标本的巨大多样性，在固定和生活条件。值得注意的是，拟议的仪器将使独特的能力和急需的额外的共焦能力提供给整个凯克用户群和更大的华盛顿大学研究社区。 为了实现这些目标，我们要求Leica SP 8 WLL共聚焦显微镜配备倒置的Leica 6000显微镜支架。该系统配备白色激光（WLL），可选择470 - 670 nm的任何激发波长（最多同时选择8个）。结合徕卡极其灵活的光谱检测，荧光成像参数是用户自定义的程度目前还没有在华盛顿大学。该系统包括两个用于荧光的混合（HyD）检测器。与标准探测器相比，这些新一代探测器具有显著提高的灵敏度、宽动态范围和低噪声特性。共振扫描仪提供捕获动态事件所需的快速成像。电动阶段和自适应聚焦校正模块包括自动采样和保持正确的重点在延时研究，分别。一个温度控制阶段插入包括活细胞实验。Leica SP 8 WLL在最近的一次演示中由Keck机构的工作人员和主要用户进行了评估，发现它满足并超过了我们的所有期望。总之，拟议的仪器将允许用户优化共焦成像参数，在目前不可能的方式，克服标本的局限性，而不是强迫实验的妥协，以克服目前的仪器限制。

DESCRIPTION (provided by applicant): We are requesting funds for the purchase of a Leica SP8 WLL confocal microscope. The instrument will be placed in the University of Washington's Keck Imaging Facility. The Keck Facility is a well- established, very heavily used facility providing confocal microscope instrumentation for 20 years, serving over 50 UW research laboratories in 2012. The facility now has two outdated and well-worn confocal microscopes, one of which is no longer supported by its manufacturer. The requested instrument is required to sustain microscope access for our group of 50 users and to provide expanded technical capabilities that are necessary to a group of 6 major users for advancing research projects ranging from dynamics within single cells to development of entire embryos. Thus, the proposed instrument will be required to analyze a vast diversity of specimens, in both fixed and living conditions. Significantly, the proposed instrument will make unique capabilities and badly needed additional confocal capacity available to the entire Keck user base and larger UW research community. It is to achieve these goals that we are requesting the Leica SP8 WLL confocal microscope with an inverted, DMI 6000 microscope stand. The system is specified with a white light laser (WLL) providing selection of any excitation wavelengths (up to 8 simultaneously) from 470 - 670 nm. Combined with Leica's extremely flexible spectral detection, fluorescent imaging parameters are user-customizable to a degree not currently available at the University of Washington. The system includes two hybrid (HyD) detectors for fluorescence. These new-generation detectors provide significantly increased sensitivity, wide dynamic range and low noise characteristics compared to standard detectors. A resonance scanner provides rapid imaging necessary for capturing dynamic events. A motorized stage and adaptive focus correction module are included for automated sampling and maintaining correct focus during time-lapse studies, respectively. A temperature-controlled stage insert is included for live-cell experiments. The Leica SP8 WLL was evaluated by the Keck Facility staff and major users at a recent demonstration and found to meet and exceed all of our expectations. In summary, the proposed instrument will permit users to optimize confocal imaging parameters, in ways currently impossible, to overcome the specimen's limitations rather than forcing experimental compromises necessary to overcome current instrument limitations.