Measurement of biomolecular interactions and association via light scattering

1.三种蛋白质，卵清蛋白，核糖核酸酶A，卵类粘蛋白，和三种蛋白质的二元混合物的光散射的浓度依赖性进行了测量，在各种组合物和pH值在总蛋白质浓度高达35克/升，条件下，核糖核酸酶承担净正电荷和其他蛋白质承担净负电荷。 在三个模型的背景下解释了浓度依赖性：统计热力学模型和有效硬颗粒模型的两个扩展，该模型被推广到允许二元混合物中物种之间存在非加性相互作用（D. Wu，中国茶条A. Minton）。 2.进行实验以确定是否可以使用荧光分光光度计代替专用光散射光度计进行定量组成梯度光散射实验。 迄今取得的结果令人鼓舞，但需要进一步开展工作，以实现必要的再现性。

1. The concentration dependence of light scattering of three proteins, ovalbumin, ribonuclease A, and ovomucoid, and binary mixtures of the three proteins were measured at various compositions and pH values at total protein concentrations of up to 35 g/l, under conditions such that ribonuclease bears a net positive charge and the other proteins bear a net negative charge. The concentration dependence was interpreted in the context of three models: a statistical thermodynamic model and two extensions of the effective hard particle model generalized to allow for the presence of non-additive interactions between species in a binary mixture (D. Wu, A. Minton). 2. Experiments were performed to determine whether quantitative composition gradient light scattering experiments can be performed using a fluorescence spectrophotometer in place of a dedicated light scattering photometer. Results obtained so far are encouraging, but further work will be required in order to achieve the requisite reproducibility.