Integrin Signaling Pathways in Prostate Cancer

描述(由申请人提供)：整合素组成一个细胞表面受体大家族，在癌细胞增殖、侵袭和转移潜力中发挥关键作用。在上一个资助周期中进行的实验发现了一种由av？6整合素介导的前列腺癌进展的新途径。我们发现av？6在人和小鼠正常前列腺中不表达，但在癌前病变、前列腺癌(ADCA)和骨转移中显著上调。从机制上讲，av？6的表达通过JNK介导的途径导致雄激素受体(AR)活性上调。我们的发现首次表明，整合素可以调节AR的活性。在这个途径中，我们发现Survivin是一种双功能的细胞分裂调节因子和细胞凋亡抑制因子，是一种关键的下游效应分子，在表达av？6的细胞中上调。我们的体内数据表明，与不同的av整合素av？3相比，av？6的表达可以促进肿瘤的生长和转移。av？6的表达还导致肿瘤介导的骨溶解，这是前列腺癌转移的关键步骤。由于av？3已被证明在前列腺癌转移灶中促进骨生长，我们假设骨病变的形成程度由av？6和av？3的相对表达水平控制。最后，我们证明了这两种整合素的表达受两种不同的转录因子的调节：av？6是通过Smad3的激活诱导的，而av？3的表达是由Runx2介导的。我们提出了一个统一的假设，即av？6整合素作为多个信号通路的整合因子，促进前列腺癌进展的早期和晚期。这一假设将在目前的续签申请中得到检验，目的如下。在目标1中，将研究av？6激活AR的分子机制。在目标2中，我们将在体内通过前列腺癌的小鼠模型来研究av？6在前列腺癌进展中的作用；为此，我们将使用发生PIN、癌症和转移的前列腺特异性Pten缺失小鼠模型。在目标3中，为了证明av整合素作为前列腺癌骨损伤治疗的分子靶点，我们将剖析av？6和av？3在体内促进骨病的途径。我们的长期目标是利用这一新产生的知识来开发前列腺癌进展的新型分子拮抗剂。

DESCRIPTION (provided by applicant): Integrins comprise a large family of cell surface receptors with critical roles in cancer cell proliferation, invasion and metastatic potential. Experiments carried out during the last funding cycle have uncovered a novel pathway of prostate cancer progression mediated by the av¿6 integrin. We have found that av¿6 is not expressed in human and mouse normal prostate but becomes significantly upregulated in preneoplastic lesions, prostatic adenocarcinoma (AdCa) and bone metastasis. Mechanistically, av¿6 expression results in upregulation of androgen receptor (AR) activity via a JNK-mediated pathway. Our findings show, for the first time, that integrins modulate AR activity. In this pathway, we have identified survivin, a bifunctional regulator of cell division and inhibitor of apoptosis, as one of the critical downstream effector molecule which becomes upregulated in cells expressing av¿6. Our in vivo data demonstrate that expression of av¿6 results in enhanced tumor growth and metastatic dissemination as compared to a different av integrin, av¿3. Expression of av¿6 also causes tumor-mediated osteolysis, a crucial step in prostate cancer metastasis. Since av¿3 has been shown to promote bone gain in prostate cancer metastatic lesions, we hypothesize that the extent of bone-lesion formation is controlled by the relative expression levels of av¿6 and av¿3. Finally, we show that these integrins' expression is regulated by two different transcription factors activated by TGF¿: av¿6 is induced via Smad3 activation, whereas av¿3 expression is mediated by Runx2. We have formulated a unifying hypothesis that av¿6 integrin functions as an integrator of multiple signaling pathways to promote early and late phases of prostate cancer progression. This hypothesis will be tested in the present renewal application by the following aims. In Aim 1, the molecular mechanisms by which av¿6 activates AR will be investigated. In Aim 2, we will investigate in vivo the role of av¿6 in prostate cancer progression in a mouse model of prostate cancer; for this purpose, we will use the prostate specific Pten-null mouse model which develops PIN, cancer and metastasis. In Aim 3, to credential av integrins as molecular targets in prostate cancer bone lesion therapies, we will dissect the pathways by which av¿6 and av¿3 contribute to bone disease in vivo. Our long-term objective is to take advantage of this newly generated knowledge to develop novel molecular antagonists of prostate cancer progression.