Animal Models and Tissue Engineering Core

动物模型与组织工程核心 皮肤是一个复杂的器官，通过空间编程的生长和分化进行终身更新。它由多种不同的细胞类型(上皮细胞、成纤维细胞、内皮细胞、脂肪细胞、免疫细胞、神经元等)组成。它们协同工作以允许正常的生理或被破坏以产生病理。 皮肤的正常和疾病状态都以特定的三维结构为特征 分离培养细胞的这些细胞类型之间的相互作用不能完全概括。基础和翻译研究的一个关键方面涉及皮肤组织和动物模型的建立和应用，这些模型具有完整的表皮通透性屏障和与体循环的生理联系。动物模型和组织工程核心的目标是提供广泛的动物和皮肤组织模型的咨询、生成和数据分析。这包括1)3D器官型皮肤培养， 2)转基因人表皮和真皮细胞在免疫缺陷小鼠上的新生人类皮肤再生；3)皮损或正常皮肤移植到免疫缺陷小鼠上；4)各种手术和生理性的小鼠和猪创伤愈合模型；5)皮肤细胞靶向基因工程小鼠模型。这些模型将针对每个研究人员在功能、机制和药理学研究方面的具体需求而量身定做。我们的服务将通过基础科学研究人员、皮肤科医生和外科医生的团队努力提供。这些核心资源不仅将加强对当前皮肤的研究，而且还将促进其他研究人员进入皮肤研究领域，这些研究人员缺乏发起此类努力的技术基础，因为历史上关注其他系统。我们相信，杜克大学研究界有强烈的愿望和巨大的能力来扩大其以皮肤病为重点的研究。

Animal Models and Tissue Engineering Core The skin is a complex organ that undergoes life-long renewal through spatially programmed growth and differentiation. It is composed of many distinct cell types (epithelial, fibroblast, endothelial, adipose, immune, neurons, etc.) that work in concert to allow normal physiology or that are disrupted to yield pathologies. Both normal and disease states of the skin are characterized by a specific three-dimensional architecture and interactions between these cell types which cultured cells in isolation cannot fully recapitulate. A critical aspect of basic and translational research involves the generation and application of skin tissue and animal models with an intact epidermal permeability barrier and physiologic connections to the systemic circulation. The goal of the Animal Models and Tissue Engineering Core is to provide consultation, generation and data analyses of a broad range of animal and skin tissue models. These include 1) 3-D organotypic skin culture, 2) de novo human skin regeneration on immunodeficient mice with genetically modified human epidermal and dermal cells, 3) lesional or normal human skin xenotransplantation onto immunodeficient mice, 4) various surgical and physiological wound healing models of mouse and swine, and 5) mouse models with skin cell targeted genetic engineering. These models will be tailored towards the specific needs of each investigator for functional, mechanistic and pharmacological studies. Our service will be provided through team efforts of basic science researchers, dermatologists and surgeons. These core resources will not only strengthen the investigations of current skin but also facilitate entry into skin research for other investigators who lack the technical basis to initiate such effort given a historical focus in other systems. We are confident that Duke research community has a strong desire and an enormous capacity to expand its skin disease-focused research.