Electrophysiology

电生理学核心将为电生理学研究提供集中服务，包括体外脑切片的制备、膜片钳记录、免疫组织化学以及相关的图像和数据分析。项目3（Scammell）和项目5（Chamberlin）将使用该核心设施。项目3将使用核心的服务来研究食欲素和强啡肽如何增加前脑活动。具体而言，这些实验将定义突触前和突触后效应以及这些神经递质影响基底前脑皮质投射神经元的机制。我们还将确定参与这些反应的肽受体和电导。项目5将使用电生理学核心来确定Kolliker-Bronze（KF）核的神经元是否具有化学敏感性。我们将确定哪些KF神经元对pH值的变化有内在的响应，并确定这些响应的机制。这些项目将受益于核心的专门知识和许多共享资源。

The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.