In Vivo Microsurgical Anastomosis of Prevascularized Tissues

描述（由申请人提供）：工程组织血管化的两种主要方法——血管生成和血管生成——需要几天才能在植入的构建体中产生灌注。这项R03试点研究旨在开发第三种方法，将预血管化的微流控组织与宿主血管进行显微外科吻合，为灌注提供了直接的物理途径。本工作将以大鼠股动脉和股静脉为受体，植入内皮化的微流控胶原和纤维蛋白组织，并分析其灌注维持情况和组织活力。该结构将密集地播种脂肪细胞，以模拟软组织替代物。在这种血管生成的物理策略能够实现其作为血管生成和血管生成替代方案的潜力之前，这项原理验证研究是重要的必要步骤。这项工作将为生成血管化组织提供广泛的支持工具，并为工程血管化脂肪组织提供具体途径，用于治疗肿瘤切除或重大创伤后常见的大型软组织缺损。

DESCRIPTION (provided by applicant): The two dominant approaches to vascularization of engineered tissues-angiogenesis and vasculogenesis-require several days to generate perfusion in implanted constructs. This proposed R03 pilot study seeks to develop a third approach, in which microsurgical anastomosis of a prevascularized microfluidic tissue to host vessels provides an immediate, physical route to perfusion. This work will implant endothelialized microfluidic collagen- and fibrin-based tissues using the rat femoral artery and vein as recipients, and will analyze the maintenance of perfusion and tissue viability in the construct. The constructs will be densely seeded with adipocytes to model soft tissue replacements. This proof-of-principle study is an important requisite step before such a physical strategy for vascularization can realize its potential as an alternative to angiogenesis and vasculogenesis. This work will provide a broad enabling tool for generating vascularized tissues and a concrete path to engineering vascularized adipose tissue for treatment of large soft tissue defects, as is often seen after tumor resection or major trauma.