Seahorse XF96e Metabolic Analyzer purchase

描述(由申请人提供)：本建议书要求为一台海马XF96e代谢分析仪提供资金。该系统将安装在威斯康星大学碳骨癌中心(UWCCC)威斯康星医学研究所的小分子筛选和合成设施中。它将向UWCCC的成员开放，更广泛地向UW医院和药房大楼的研究人员开放。虽然到目前为止已经在全国范围内售出了大约600台分析仪，但这将是威斯康星大学医学院和公共卫生校园里的第一台分析仪。它是一种独特的仪器，具有补充或取代目前在我们生物医学研究实验室中运行的分析的潜力。该分析仪生成关于糖酵解和氧化磷酸化的相对速率的详细信息，无论是在平衡状态下，还是在活细胞系统扰动之后。随着新陈代谢重新成为生长的关键调节因素，不仅是肿瘤，而且是干细胞群体，这一仪器将成为解释其表型的关键。

DESCRIPTION (provided by applicant): This proposal requests funds for a Seahorse XF96e Metabolic Analyzer. The system will be housed in the Small Molecules Screening and Synthesis Facility at the Wisconsin Institutes for Medical Research in the University of Wisconsin Carbone Cancer Center (UWCCC). It will be available for members of the UWCCC, and more widely, for researchers in the UW Hospital and Pharmacy buildings. Although there have been some 600 Analyzers sold nation-wide to date, this will be the first analyzer to come to the University of Wisconsin School of Medicine and Public Health campus. It represents a unique instrument with the potential to complement or replace the assays that are currently run in our biomedical research labs. This analyzer generates detailed information about the relative rates of glycolysis and oxidative phosphorylation, either at equilibrium, or after perturbation of live cell systems. With the re-emergence of metabolism as a key regulator of growth, not only of tumors, but stem cell populations, this instrument will be key to the interpretation of their phenotypes.