Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches

项目1：解决三叉神经支受压引起的口面部神经性疼痛 使用合理整合的互补性方法 摘要 由三叉神经感觉神经元支配的颅面部神经病理性疼痛大约在 至少1x106个美国人。该项目的目标是评估效率并阐明 合理组合辅助方法治疗三叉神经病理性疼痛。中心假说 项目-1的特点是天然促分解脂调节剂(专门的促分解脂调节剂，SPM)或 耳穴电针(AEA)在减轻口面部神经性疼痛方面具有良好的疗效， 通过调节神经炎症和神经可塑性而因压力而加重。我们将使用一个久负盛名的 建立小鼠三叉神经病理性疼痛模型，慢性眶下神经缩窄(CCI-ion)，以检测 我们的核心假设。我们将首先定义神经病理性疼痛和炎症的时间进程 有应激和无应激的两性对CCI-离子反应的神经炎性特征。随后，我们 将确定AEA和SPM对疼痛相关行为以及炎症消退的影响 CCI-离子诱发的神经炎性改变。最后，我们将探讨AEA和SPM的有效性 降低三叉神经感觉神经元和持续痛激活的中枢神经系统神经元的自发活动 在CCI-ION之后。

Project 1: Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches ABSTRACT Neuropathic pain in the craniofacial region innervated by trigeminal sensory neurons affects approximately at least 1x106 Americans. The goal of this project is to assess efficiency and elucidate key mechanisms of rationally-combined complementary approaches to treat trigeminal neuropathic pain. The central hypothesis of Project-1 is that natural pro-resolution lipid mediators (specialized pro-resolution lipid mediators, SPMs) or auricular electro-acupuncture (aEA) can produce beneficial effects in alleviating orofacial neuropathic pain, aggravated by stress, by modulating neuroinflammation and neural plasticity. We will use a well-established mouse model of trigeminal neuropathic pain, chronic constriction of the infraorbital nerve (CCI-ION), to test our central hypothesis. We will first define the time course of neuropathic pain and inflammatory and neuroinflammatory profiles in response to CCI-ION in both sexes with and without stress. Subsequently, we will determine the effects of aEA and SPMs on pain-related behaviors as well as resolution of inflammatory and neuroinflammatory profiles evoked by CCI-ION. Finally, we will explore effectiveness of aEA and SPMs on reducing spontaneous activity of trigeminal sensory neurons and of ongoing pain-activated CNS neurons after CCI-ION.