UCSF DVS CyTOF Mass Cytometer

描述(由申请人提供)：加州大学旧金山分校(UCSF)正在申请资金，用于购买DVS Sciences公司的CyTOF C5质量细胞仪，该仪器配备了Cytobank高级数据管理和分析软件。质量细胞术是一项变革性的新技术，它在加州大学旧金山分校是不可用的，它允许识别和研究罕见的疾病细胞。CyTOF质谱仪将原子质谱仪的分辨率、特异度和灵敏度与目前由流式细胞仪提供的高通量单细胞分析优势结合在一起。使用稳定的同位素纯(非放射性)金属标签，每个单个细胞可以同时识别和量化多达40个参数，产生独特的生物标记物签名，区分更精细的细胞群体分析。DVS Sciences是唯一一家质量细胞仪制造商。这一应用的推动力是加州大学旧金山分校缺失的质量细胞仪功能。来自加州大学旧金山分校12个不同部门的21名研究人员的43个科学项目为资助提供了理由。收购CyTOF质量细胞仪将创建一个共享资源，预计每年使用超过2500个小时，表明对这项技术的真正需求。四名研究人员目前正在通过低效的远程合作使用这项技术。临床活检和人体样本占该系统估计使用量的80%以上，潜在的生物危害在其他机构不易适应。为了满足这一技术需求，加州大学旧金山分校耗资200万美元的翻新项目正在建造一个指定的BSL2(生物安全级别)房间，用于大规模细胞分析资源。这一资源将是现有核心设施的一部分，包括构建新型金属标记探针、管理CyTOF仪器操作、使用复杂的软件工具管理和分析多参数质量细胞仪数据、为用户群提供持续培训和支持，以及为加州大学旧金山分校的学生提供教育课程。机构支持将占CyTOF M5质量细胞仪最终成本的17%。

DESCRIPTION (provided by applicant): The University of California, San Francisco (UCSF) is requesting funds for the purchase of a CyTOF C5 Mass Cytometer by DVS Sciences with Cytobank advanced data management and analytical software. Mass cytometry is a transformational new technology unavailable at UCSF that permits the identification and investigation of rare, diseased cells. The CyTOF mass cytometer marries the resolution, specificity, and sensitivity of atomic mass spectrometry to the high-throughput, single-cell analytical advantages currently provided by flow cytometry. Using stable isotopically pure (non-radioactive) metal tags, as many as forty parameters per single cell can be identified and quantified simultaneously, yielding unique biomarker signatures that distinguish a more refined analysis of cell populations. DVS Sciences is the only mass cytometer manufacturer. The impetus for this application is UCSF's missing mass cytometer capability. There are 43 scientific projects by 21 investigators from 12 different UCSF departments providing justification for funding. Acquisition of the CyTOF mass cytometer will create a shared resource with a projected usage over 2500 hours per year, demonstrating a real need for this technology. Four investigators are currently using the technology through inefficient long-distance collaborations. Clinical biopsies and human samples represent over 80% of the estimated usage of this system, potential biohazards not easily accommodated at other institutions. In response to this technology need, UCSF in a $2M renovation project is building a designated BSL2 (biosafety level) room for a mass cytometry resource. This resource will be part of an existing core facility and includes the ability to construct novel metal-tagged probes, manage CyTOF instrument operation, employ sophisticated software tools for managing and analyzing multi-parametric mass cytometer data, offer continued training and support to the user base, and educational courses to UCSF students. Institutional support will total 17% of the final cost for the CyTOF M5 Mass Cytometer.