Laminar Organization of Cortical Functional Connectivity in Monkeys

在需要注意和工作记忆的任务中，前额叶和顶叶皮质表现出功能性连接 以振荡锁相的形式。项目4将测试锁相依赖于 关于起源于第三层神经元的两个区域之间的直接联系。通过监测脑电 从皮质表面到白质的微小间隔内同时活动，而猴子参与其中 在工作记忆(目标1)和注意力(目标2)中，实验将检验特定的预测符号 的功能连接性在第三层最大。通过监测识别的投射的电活动 通过检查两个区域之间的阻挡投射的影响，实验将 测试直接投影对功能连接至关重要的具体预测(目标3)。结果 在项目4中得到的结果将与关于认知起源的中心假说直接相关 精神分裂症中的偏执症。这一假说指出，认知缺陷的产生是因为 第三层锥体细胞干扰了前额叶和顶叶皮质之间的功能连接。项目 1-3将专注于健康大脑和精神分裂症患者第三层锥体细胞的特性。项目5 将重点放在健康大脑和精神分裂症患者的功能连接上，这是通过一致性来衡量的 和因果关系分析。项目4的独特贡献将是通过以下方式将这些查询领域联系起来 用一致性和因果关系评估第三层神经元在功能连通性中的作用 分析。 相关性(请参阅说明)： 这个项目将确定第三层神经元在精神分裂症认知偏转中的作用。

In tasks requiring attention and working memory, prefrontal and parietal cortex exhibit functional connectivity in the form of oscillatory phase-locking. Project 4 will test the general hypothesis that phase-locking depends on direct connections between the two areas originating from neurons in layer 3. By monitoring electrical activity simultaneously at fine intervals from the cortical surface to the white matter, while monkeys engage in working memory (Aim 1) and attenfion (Aim 2), the experiments will test the speciflc predicfion that signs of funcfional connectivity are maximal in layer 3. By monitoring the electrical activity of identified projection neurons and by examining the impact of blocking projections between the two areas, the experiments will test the specific prediction that direct projections are critical for functional connectivity (Aim 3). Results obtained in Project 4 will have direct relevance to the Central Hypothesis regarding the origin of cognitive deflcits in schizophrenia. This hypothesis states that cognifive deficits arise because pathological changes in layer 3 pyramidal cells interfere with functional connectivity between prefrontal and parietal cortex. Projects 1-3 will focus on the properties of layer 3 pyramidal cells in the healthy brain and in schizophrenia. Project 5 will focus on funcfional connectivity in the healthy brain and in schizophrenia as measured with coherence and causality analyses. The unique contribufion of Project 4 will be to link these domains of inquiry by establishing the role of layer 3 neurons in functional connectivity as assessed with coherence and causality analyses. RELEVANCE (See instructions): This project will establish the role of layer 3 neurons in the cognitive deflcits in schizophrenia.