A new approach to quantitative, point-of-care serology

描述（由申请人提供）：我们最近展示了一种无试剂的电化学方法-称为E-DNA传感器-可以直接在未稀释，未处理的人血清中检测抗hiv抗体，其浓度比hiv阳性患者样本中的浓度低几个数量级。该方法快速（10分钟以下），单步和定量，从而提高了现有的点护理分子诊断的便利性和/或临床价值。进一步说明其潜在价值，该方法支持微米级电极，因此可以复用到在单个手指刺破样本中测量数十种诊断抗体的水平。考虑到这些属性，我们的技术似乎非常适合于在中心实验室之外从定量测量多种抗体水平的能力中获得价值的应用。拟议研究计划的重点是通过对E-DNA抗体检测平台进行初步的临床前验证来验证这一假设。具体来说，我们将制作E

DESCRIPTION (provided by applicant): We have recently demonstrated a reagentless, electrochemical method - termed E-DNA sensors - that achieves the detection of anti-HIV antibodies directly in undiluted, unprocessed human blood serum at concentrations orders of magnitude lower than those seen in HIV-positive patient samples. The approach is rapid (sub-10 min), single-step, and quantitative, thus improving on the convenience and/or clinical value of existing point-of-care molecular diagnostics. Further speaking to its potential value, the approach is supported on micron-scale electrodes and can thus be multiplexed to the level of measuring dozens of diagnostic antibodies in a single finger-prick sample. Given these attributes, our technology appears well suited for applications that would derive value from the ability to measure quantitatively the levels of multiple antibodies outside of central laboratories The focus of the proposed research program is to test this hypothesis by performing the initial, pre-clinical validation of the E-DNA antibody detection platform. Specifically, we will fabricate E DNA arrays for the measurement of one to two dozen antibodies diagnostic of a panel of three sexually transmitted infections, and perform side-by-side comparison of these against current gold standard laboratory approaches when both are challenged using authentic human samples.