Immunology Core

为了了解土拉方杆菌(Francisella tularsis，Ft)的免疫应答及其与免疫细胞的相互作用， 细胞和组织的组织学和免疫组织化学分析，以及对个体 免疫细胞群和信号分子的产生是必需的。因此，核心C的目的是 为研究人员提供分析免疫细胞、量化信号的手段和技术专长 分子(细胞因子和细胞内信号分子)和组织学识别细胞和组织 从老鼠身上获得。这包括使用免疫组织化学技术来定位和识别 免疫细胞表面和内部的抗原。这一核心设施将由三个主要部分组成 组件：(1)Flow Cvtometrv服务，(2)Bio-Plex多重阵列(BMA)蛋白质检测服务。 (3)组织化学/免疫组织化学服务。所有这三项服务目前都可用，并且正在 就目前资助的所有3个次级项目向P01调查人员提供。关于目前的提议，核心C 将继续就所有拟议次级项目向P01调查员提供上述服务。的功能 以下是免疫学的三个核心组成部分：1.流式细胞术服务：这 组件将为调查人员提供对流式细胞仪分析和细胞分类的访问，目的是 分析单个免疫细胞群体，包括表面标记表达和细胞内细胞因子 制作。具体职能包括：流式细胞仪操作、调查人员培训、设备 维护、订购试剂和用品；2.BMA蛋白质检测服务：该组件包括 利用BioRad销售的成熟的BMA技术来量化分泌的细胞因子和细胞内 利用专门用于此目的的Bio-Plex 200仪器向分子发送信号。具体功能将| 包括：获取试剂和试剂盒，进行化验，进行样本分析，协助 适当时进行数据/化验解释，并维护所需的设备； 组织学/免疫组织化学服务：该组件将处理由 组织学和免疫组织化学评估的研究人员。功能将包括组织嵌入和 细胞和组织的切片、组织化学和免疫组织化学染色、设备维护 以及在数据解释方面的协助。核心C现在还将生产、测试和标准化停用的Ft (Ifo-mAb复合体，将由所有3个次级项目使用。

To understand the immune response to Francisella tularensis (Ft), and its interaction with immune cells, histological and immunohistochemical analysis of cells and tissues, as well as an analysis of individual immune cell populations and signaling molecules produced, are required. Thus, the purpose of Core C is to provide the means and technological expertise for investigators to analyze immune cells, quantify signalling molecules (cytokines and intracellular signaling molecules), and histologically identify cells and tissues obtained from mice. This includes the use of immunohistochemical techniques to localize and identify antigens on the surface of, and inside immune cells. This Core facilitv will be composed of three primary components: (1) a Flow Cvtometrv service, (2) a Bio-Plex Multiplex Array (BMA) Protein Detection service. and (3) a Histochemistry/lmmunohistochemistry service. All three services are currently available, and being provided to P01 investigators on all 3 currently funded subprojects. In regard to the current proposal, Core C will continue to provide the above services to P01 investigators on all proposed subprojects. The function of the three Immunology Core components is indicated in more detail below: 1. Flow Cytometry Service: This component will provide investigators access to flow cytometry analysis and cell sorting for the purpose of analyzing individual immune cell populations, including surface marker expression and intracellular cytokine production. Specific functions will include: flow cytometer operation, investigator training, equipment maintainance, ordering of reagents and supplies; 2. BMA Protein Detection Service: This component wi utilize an established BMA technology sold by BioRad to quantitate secreted cytokines and intracellular signalling molecules utilizing a Bio-Plex 200 instrument dedicated to this purpose. Specific functions wil| include: acquiring reagents and kits, performing assays, performing analysis of samples, assisting in data/assay interpretation when appropriate, and maintaining the required equipment; 3. Histology/lmmunohistochemistry Service: This component will process cells and tissues provided by investigators for histologic and immunhistochemical evaluation. Functions will include tissue embedding and sectioning, histochemical and immunohistochemical staining of cells and tissues, equipment maintainance and assistance in the data interpretation. Core C will now also produce, test, and standardize inactivated Ft (iFO-mAb complexes, which will be used by all 3 subprojects.