120kV Transmission Electron Microscope for a Multi-user Microscopy Facility

描述(申请人提供)：高分辨率形态分析是生理和病理条件下生物学研究的一个组成部分。在细胞运动或细胞分泌过程中诱导的细微结构改变被高分辨率地用透射电子显微镜(TEM)分析来捕捉。同样，在不同的实验条件下观察到的亚细胞器(晚期内质体和溶酶体)的形态变化可以通过透射电子显微镜分析得到强有力的可视化。对细菌和真菌的分析可以提供关于它们在不同实验条件下的形态变化的非常有用的信息。本申请的主要用户正在申请资助最先进的高分辨率透射电子显微镜和相关的数码相机。所要求的瞬变显微镜将是分析成像设施(AIF)的一部分，这是一个多用户成像资源，可供所有训练有素的调查人员使用。目前，一个老化的JEOL JEM-100CX瞬变显微镜安装在1982年，存在于设施中，不能满足这些研究人员的需要。在这一应用中，主要用户的共同科学需求是能够在不同的实验条件下获得细胞、细胞器或病原体的高分辨率成像。具有高亮度LaB6电子源和CCD摄像机的新型透射电子显微镜将实现高分辨率成像和数字数据记录。这项建议中描述的透射电子显微镜将允许组装的主要用户分析病原体的形态；内体生物发生和蛋白质分布的精细结构方面；以及否则将被排除的颗粒分泌。

DESCRIPTION (provided by applicant): High resolution morphological analysis is an integral part of biological investigations under both physiological and pathological conditions. The fine structure modifications induced during cell motility or cellular secretion are captured in high resolution by transmission electron microscopy (TEM) analysis. Similarly, morphological changes observed in sub-cellular organelles (late endosomes and lysosomes) during various experimental conditions can be powerfully visualized by TEM analysis. Analysis of bacteria and fungi can provide very useful information on their morphological changes under differential experimental conditions. The Major Users of this application are requesting funding of a state of the art high resolution transmission electron microscope and an associated digital camera. The requested TEM will be part of the Analytical Imaging Facility (AIF), a multi user imaging resource, and available to all trained investigators. Currently, an aging JEOL JEM-100CX TEM installed 1982, is present in the facility, does not fulfill the needs of these investigators. The common scientific need of the major users in this application is the ability to obtain high resolution imaging of cells, organelles or pathogens under different experimental conditions. A new TEM with a high brightness LaB6 electron source and CCD camera will enable high resolution imaging and digital data recording. The transmission electron microscope described in this proposal will allow the assembled major users to analyze pathogen morphology; fine structural aspect of endosomal biogenesis and protein distribution; and granule secretion which will be otherwise precluded.