Microscopy

项目总结(见说明)： 显微镜共享资源(MSR)是一个位于中央的资源，拥有尖端仪器和训练有素的专家，为OSUCCC的科学家提供共焦、光学、扫描电子和透射电子显微镜方面的杰出支持。MSR拥有两台电子显微镜和四台共焦显微镜，自2005年以来都是通过联邦拨款和杰出的机构支持购买的。这些仪器包括两台奥林巴斯FVI 000单光子共聚焦显微镜，每个显微镜都有四束激光和专门针对固定细胞和组织的高NA物镜；一台FEI Tecnai BioTwin透射式电子显微镜；以及一台奥林巴斯FVI 000多光子共聚焦仪器和迈泰DeepSee激光，可以深入探测活体动物和固定组织中的肿瘤。MSR由Richard Burry博士领导，他是一位在显微镜领域拥有30多年丰富经验的老牌科学家和资金雄厚的科学家，他与经验丰富和训练有素的工作人员一起，为OSUCCC研究人员提供实验设计和图像分析方面的重要咨询。通过组织良好的培训课程和工作人员提供的个人培训，MSR的使用率和生产率得到了提高。MSR被广泛用于OSUCCC的科学项目，为OSUCCC的30个成员实验室提供服务，为高质量的癌症相关出版物和赠款生成图像。MSR位于生物医学研究塔(BRT)的二楼，靠近OSUCCC成员的实验室。基于MSR能力的扩展和OSUCCC成员的赠款申请数量的增加，基于战略招聘目标和 癌症研究中对高端显微镜的需求扩大。MSR得到了杰出的机构资源的支持，利用与俄亥俄州大学学院、俄亥俄州研究办公室、俄亥俄州和俄亥俄州SUCCC的广泛合作伙伴关系。MSR是一种新的OSUCCC共享资源，以前拥有强大的用户基础作为OSU的核心设施，从而实现了NIH整合核心设施的目标，以最大限度地提高NIH资助的研究人员的效率和利用率。总的来说，MSR是一个关键 OSUCCC研究人员共享资源，寻求识别正常组织和肿瘤中的特定细胞和蛋白质，以增强我们在制定治疗策略时对癌症基本过程的理解。

PROJECT SUMMARY (See instructions): The Microscopy Shared Resource (MSR) is a centrally-located resource with cutting-edge instruments and highly trained experts to provide outstanding support for OSUCCC scientists for confocal, light, scanning electron and transmission electron microscopy, the MSR has two electron microscopes and four confocals all purchased since 2005 through federal grants and outstanding institutional support. These instruments include two single photon Olympus FVI 000 confocal microscopes each with four lasers and high N.A. objectives specifically for fixed cells and tissue, an FEI Tecnai BioTwin transmission electron microscope, and an Olympus FVI 000 multiphoton confocal instrument with a MaiTai DeepSee laser to probe deep into tumors in both live animals and fixed tissue. The MSR is led by Dr. Richard Burry, an established and well funded scientist with over 30 years of extensive expertise in microscopy, who along with an experienced and highly-trained staff, provides OSUCCC investigators vital consultation in experimental design and image analysis. Usage and productivity from the MSR is enhanced by well-organized training courses and individual training offered by staff members. The MSR is extensively used across OSUCCC scientific programs providing service to >30 OSUCCC member labs to generate the images for high quality cancer relevant publications and grants. The MSR is centrally located on the second floor of the Biomedical Research Tower (BRT) close to the labs of the OSUCCC members. Based on the expanding capabilities of the MSR and increases in number of grant applications from OSUCCC members, the OSUCCC usage is estimated to increase dramatically over the next five years based on strategic recruitment goals and expanded demand for high-end microscopy in cancer research. The MSR is supported by outstanding institutional resources by leveraging extensive partnerships with OSU Colleges, the OSU Office of Research, grants from the State of Ohio and the OSUCCC. The MSR is a new OSUCCC shared resource, previously with a strong user base as an OSU core facility, and thus fulfills NIH goals to consolidate core facilities for maximal efficiency and utilization by NIH funded investigators. Collectively the MSR is a critical shared resource for OSUCCC investigators seeking to identify specific cells and proteins in normal tissue and in tumors to enhance our understanding of fundamental processes of cancer in developing therapeutic strategies.