Acquisition of Octet Biolayer Interferometry system for MIT biophysics facility

项目摘要 所有的生命过程都是由蛋白质与小分子之间的动态相互作用来调节的 配体、伴侣蛋白、核酸或其它大分子。仪器能够 对这些体外相互作用的特异性和亲合力的定量分析提供了 理解复杂的反应网络的物理基础， 复杂的生物过程。在这项提案中，生物学系和 麻省理工学院的生物工程部门申请资金购买Octet RED 96仪器 用于生物分子相互作用的分析。这种仪器利用生物层 干涉测量（BLI）来提供关于溶液中配体与配体结合的真实的时间信息， 固定化分子仪器采用96孔板格式，用于高浓度 通量分析，并且非常通用。配体可以是小分子 （>150 Da）至非常大的蛋白质或核酸寡聚体，以及缔合的获得 和解离速率常数迅速提供了广泛的动态平衡常数 范围（10 pM - 1 mM），具有非常有效的样品要求。社区获得实践机会 使用这种设备将提高多个研究项目的速度和复杂性 并将通过共享生物物理中仪器的中央位置来保证 仪器设施（BIF）在麻省理工学院校园68号楼，这是由一个全职运行 技术员（Debby Pheasant女士），由Barbara Imperiali教授指导。监督 负责采购和管理BIF所有设备的委员会包括 教授芭芭拉Imperiali（主席），罗伯特绍尔，艾米E。Keating，Jacquin奈尔斯和Ms. 黛比·菲森特 !

PROJECT SUMMARY All living processes are regulated by the dynamic interactions of proteins with small molecule ligands, partner proteins, nucleic acids, or other macromolecules. Instrumentation that enables quantitative analysis of the specificities and avidities of these interactions in vitro provides a physical foundation for understanding the intricate networks of reactions that contribute to complex biological processes. In this proposal, eleven faculty in the Departments of Biology and Biological Engineering at MIT request funding for the acquisition of an Octet RED96 instrument for the analysis of biomolecular interactions. This instrumentation exploits biolayer interferometry (BLI) to provide real time information on the binding of ligands in solution to immobilized molecules. The instrumentation accommodates a 96-well plate format for high- throughput analysis and is exceptionally versatile. Ligands can range from small molecules (>150 Da) up to very large protein or nucleic acid oligomers, and the acquisition of association and dissociation rate constants rapidly provides equilibrium constants over a broad dynamic range (10 pM - 1 mM) with very efficient sample requirements. Community access to hands-on use of this equipment will advance the pace and sophistication of multiple research programs and will be guaranteed by the central location of the instrument in the shared Biophysical Instrumentation Facility (BIF) in Building 68 of the MIT campus, which is run by a full-time technician (Ms. Debby Pheasant) and is directed by Professor Barbara Imperiali. The oversight committee for the acquisition and administration of all equipment in the BIF comprises Professors Barbara Imperiali (Chair), Robert Sauer, Amy E. Keating, Jacquin Niles and Ms. Debby Pheasant. !