2014 Protein Transport Across Cell Membrane Gordon Research Conference and Gordon

描述(由申请人提供)：本提案请求支持3月8日至14日在德克萨斯州加尔维斯顿加尔韦斯酒店举行的2014年蛋白质跨细胞膜传输戈登研究会议(GRC)和戈登研究研讨会(GRS)。蛋白质转运机制的阐明仍然是现代细胞生物学的一个基本目标，因为大约30%的蛋白质是通过细胞膜转运或整合到细胞膜中的。通过这些机制实现正确的细胞区划对细胞功能至关重要，而蛋白质转运的不同方面直接影响人类健康。许多遗传性疾病是由蛋白质转位或膜蛋白插入缺陷引起的；感染性微生物通过各种蛋白质运输系统运送毒力因子；目前大多数药物以分泌或膜蛋白为靶点。膜蛋白结构的爆炸、高通量遗传分析的使用、深度测序的出现以及超分辨率显微镜的进步推动了蛋白质运输领域的快速发展。因此，一次专门的蛋白质运输会议，召集不同方法的专家并采用各种实验系统，对于继续取得进展至关重要。这次会议是美国唯一一次致力于深入报道这一研究领域的定期会议。2014年会议将以该领域知名领导人的概述演讲开始，突出统一的概念、主要的实验系统和新兴的研究领域。随后的八次会议将按研究主题组织，每一次都将包括采用不同方法的演讲。这种跨学科的并置寻求在每个领域内激发新的研究方向。研究主题将包括：1)蛋白质对不同细胞内膜的分选和靶向；2)蛋白质通过转位通道的传递；3)膜蛋白质插入和组装的机制；4)运输机器的结构生物学；5)特殊的致病运输途径；6)细胞器的生物发生等。每一次会议的讨论负责人和演讲者都将包括知名领导人和早期职业科学家。组织者还热衷于培养这一领域的下一代科学家。因此，我们将继续博士后组织的GRS，以提高博士后和学生在陈述、讨论和与高级调查人员互动方面的技能。最近四个蛋白质转运GRC的统计数据表明 年轻女性涌入这个历史上由男性主导的领域。我们的目标是鼓励这一趋势，优先选择GRC和GRS发言人和讨论负责人。少数族裔也将优先选择谈话，以增加这一领域的多样性。 向NIH申请资金，用于为GRC演讲者和讨论领袖以及在GRS发言的特殊早期职业调查人员的注册和差旅提供部分支持。我们目标的实现将导致一次高度活跃和互动的会议，也将引领年轻和多样化的一代进入这一细胞生物学学科。

DESCRIPTION (provided by applicant): This proposal requests support for the 2014 Protein Transport across Cell Membranes Gordon Research Conference (GRC) and Gordon Research Seminar (GRS) March 8-14 at the Hotel Galvez in Galveston, Texas. Elucidation of protein transport mechanisms remains a fundamental objective of modern cell biology as ~30% of all proteins are either transported across or integrated into cellular membranes. Achieving correct cellular compartmentalization by these mechanisms is essential for cell function, and different facets of protein translocation directly impact human health. Many genetic diseases result from defects in protein translocation or membrane protein insertion; infectious microbes deliver virulence factors by a variety of protein transport systems; and most current pharmaceuticals target secreted or membrane proteins. The explosion of membrane protein structures, the use of high-throughput genetic analyses, the emergence of deep sequencing, and advances in super-resolution microscopy have fueled rapid advances in the protein transport field. Thus, a dedicated protein transport conference, assembling specialists in diverse methodologies and employing various experimental systems, is essential for continued progress. This conference is the only regularly scheduled meeting in the US devoted to an in-depth coverage of this research field. The 2014 conference will open with overview presentations by prominent leaders in the field to highlight unifying concepts, major experimental systems, and emerging research areas. Each of the subsequent eight sessions, organized by research topic, will incorporate talks that employ different methodologies. This cross-disciplinary juxtaposition seeks to stimulate new research directions within each area. Research topics will include: 1) sorting and targeting of proteins to different intracellular membranes, 2) the passage of proteins through translocation channels, 3) the mechanisms of membrane protein insertion and assembly, 4) the structural biology of transport machines, 5) specialized pathogenic transport pathways, 6) biogenesis of organelles, and others. Discussion leaders and speakers for each session will include both established leaders and early career scientists. The organizers are also keenly focused on nurturing the next generation of scientists in this field. We will therefore continue the postdoc-organized GRS to sharpen the skills of postdocs and students in presentation, discussion, and interaction with senior investigators. Statistics for the last four protein transport GRCs indicate an influx of young women into this historically male-dominated field. We aim to encourage that trend with preference for selection of GRC and GRS speakers and discussion leaders. Preference for talk selection will also be given to minorities to increase diversity in this field. Funds are requested from NIH for partial support of registration and travel for GRC speakers and discussion leaders, as well as exceptional early career investigators who speak at the GRS. Achievement of our objectives will result in a highly dynamic and interactive conference that also ushers a young and diverse generation into this discipline of cell biology.