Molecular Recognition by Bleomycin

描述（由申请人提供）：博莱霉素是一个糖肽衍生的抗肿瘤抗生素家族，最初从轮状链霉菌中分离出来。一些博莱霉素在临床上用于治疗鳞状细胞癌和恶性淋巴瘤。虽然在过去的几十年里对博来霉素进行了大量的研究，但很少有研究涉及博来霉素的两个显著特性，即它的寻瘤特性和它在生理条件下靶向DNA底物的方式。目前的项目是基于新的策略，提供(i)一种简便的方法来监测博来霉素的肿瘤寻找特性，（ii）一种方法来识别博来霉素的高亲和力核酸靶点。该项目的目标包括鉴定博来霉素中肿瘤靶向所需的结构元素，以及识别的细胞成分。此外，还提出了鉴定DNA基序的结合和切割具有特别高的效率由博来霉素。

DESCRIPTION (provided by applicant): The bleomycins are a family of glycopeptides derived antitumor antibiotics originally isolated from Streptomyces verticillus. Some of the bleomycins are used clinically for the treatment of squamous cell carcinomas and malignant lymphomas. While numerous studies of bleomycin have been carried out in the past few decades, few of these have addressed two remarkable properties of bleomycin, namely its tumor seeking properties and the way in which it targets its DNA substrates under physiological conditions. The present project is based on new strategies which provide (i) a facile method for monitoring the tumor seeking properties of bleomycin and (ii) an approach for identifying high affinity nucleic acid targets for bleomycin. Goals of the project include identification of the structural elements in bleomycin that are required for tumor targeting, as well as the cellular constituents that are recognized. Also proposed is the identification of DNA motifs that are bound and cleaved with exceptionally high efficiency by bleomycin.