The fates of duplicate genes at the subcellular level

描述（由申请人提供）：此拨款建议是一个F32，为期三年的博士后奖学金。这项研究涉及进化遗传学和细胞生物学领域。为这项研究提出的方法包括生物信息学，基因组学，选择，分子遗传学，显微镜和生物化学的签名分析。我建议利用一种新的方法来了解重复基因在亚细胞水平上的命运。我计划将重复基因的基因组分析能力与荧光标记旁系同源物的亚细胞共定位研究联合收割机结合起来。我将使用旁系融合信号的重叠量来衡量种内和种间旁系同源物之间功能的重叠。使用的基因家族参与膜运输，其蛋白质决定簇的行为在一个空间特异性的方式，将允许我相关的功能与亚细胞定位。草履虫奥雷利亚物种复合体中相对较新的全基因组重复和物种辐射，结合草履虫基因组之间的高度同线性，提供了一个强大的基因组系统，在其中进行这种分析。这项工作将进一步了解重复基因进化的进化原因和功能后果，这对进化遗传学以及我们对基本真核细胞生物学的理解都有影响。

DESCRIPTION (provided by applicant): This grant proposal is for an F32, three-year postdoctoral fellowship. The research proposed is in the fields of evolutionary genetics and cell biology. The methods proposed for this research include bioinformatics, genomics, and analysis of signatures of selection, molecular genetics, microscopy and biochemistry. I propose utilizing a novel approach to understanding the fates of duplicate genes at the subcellular level. I plan to combine the power of genomic analysis of duplicate genes with subcellular colocalization studies of fluorescently tagged paralogs. I will use the amount of overlap in signal of paralog fusions to gauge the overlap in function between both intra- and interspecies paralogs. The use of gene families involved in membrane trafficking, whose protein determinants act in a spatially specific manner, will allow me to correlate function with subcellular localization. The relatively recent whole genome duplications and species radiation within the Paramecium aurelia species complex, combined with a high degree of synteny between Paramecium genomes, offer a powerful genomic system in which to conduct this analysis. This work will further the understanding of both the evolutionary causes and the functional consequences of the evolution of duplicate genes, which has implications for evolutionary genetics as well our understanding of basic eukaryotic cell biology.