Flow Cytometry Core

这一至关重要的核心提供了常规和一致的分析脾细胞，颈部淋巴结 细胞，以及来源于CNS的驻留细胞和炎性细胞。如个别项目所述， 精确分析感染期间浸润CNS的细胞以及驻留的CNS细胞 （少突胶质细胞，星形胶质细胞和小胶质细胞）是整个计划的关键组成部分。这包括 免疫过程中表达的表面标志物、免疫调节分子和细胞因子的测量 由常驻和浸润细胞类型激活。精确识别和分离细胞类型是 这是采集和分析以及通过分选进行细胞纯化所必需的。该核心将提供质量 控制每个项目所需的各种细胞分选策略的有效性和纯度。这一核心将 此外，通过确认kjone骨髓嵌合体的重建， 以及繁殖群体的遗传表型。因此，主要功能是：1）提供例程 获取和分析各种组织中的细胞组成及其表型变化; 2）纯化细胞 用于体外基因谱分析和免疫学测定; 3）验证 骨髓重建; 4）分析PBMC用于基因分型;和5）为细胞培养提供质量控制。 分离和纯化。所有这些任务的执行实现了所有三个项目的基本功能。核心B 对完成整个项目的总体科学目标至关重要。

This critically important core provides routine and consistent analysis of spleen cells, cervical lymph node cells, and resident as well as inflammatory cells derived from the CNS. As noted in the individual projects, precise analysis of the cells infiltrating the CNS during infection, as well as the resident CNS cells (oligodendroglia, astrocytes and microglia) are key components of the overall program. This includes measurement of surface markers, immune modulatory molecules, and cytokines expressed during immune activation by both resident and infiltrating cell types. Precise identification and separation of cell types is required for both acquisition and analysis and cell purification by sorting. This Core will provide quality control for efficacy and purity of various cell sorting strategies required for each project. This Core will furthermore interact with the Animal Core (Core B) by confirming reconstitution of kjone marrow chimeras and genetic phenotypes in the breeding colonies. The major functions are thus to: 1) provide routine acquisition and analysis of cell composition and their phenotypic changes in various tissues; 2) purify cells from various mouse tissues for gene profiling and immunological assays in vitro; 3) verify the efficacy of bone marrow reconstitution; 4) analyze PBMC for genotyping; and 5) provide quality control for cell separation and purity. Performance of all these tasks fulfills essential functions for all three projects. Core B is critical to accomplishing the overall scientific goals of the entire project.