Aurora B-Induced Cytokinesis Defects in Malignant Cells

描述(由申请人提供)：该申请是一个正在进行的项目的更新，目的是了解癌症中的有丝分裂或细胞分裂缺陷。我们的目标是确定缺陷的来源及其对肿瘤发生的影响。在这一大类中，该项目将重点放在中心体扩增和多倍体的来源上。这是癌症生物学的一个新兴领域，其机制还知之甚少。在培养中生长的癌细胞和正常细胞都将被研究。我们已经证明，由于调节肌球蛋白轻链磷酸化的缺陷，癌细胞经常无法正常分裂。我们认为这是由于抑制了负责将磷酸添加到肌球蛋白轻链上的激酶。我们建议确定该激酶在肿瘤细胞中是如何被抑制的。如果了解分裂过程中的错误，我们或许能够阻止肿瘤细胞的异常增殖。

DESCRIPTION (provided by applicant): This application is a renewal of an ongoing project to understand mitotic or cell division defects in cancer. Our goals are to identify the source of the defects and their impact on oncogenesis. Within this broad category, the project will focus on the source of centrosome amplification and polyploidy. This is a newly emerging area of cancer biology and the mechanisms are poorly understood. Both cancer and normal cells grown in culture will be investigated. We have shown that cancer cells often fail to divide properly because of defects in regulatory myosin light chain phosphorylation. We believe that this is due to inhibition of the kinase responsible for adding the phosphate to the myosin light chain. We propose to determine how this kinase is inhibited in tumor cells. If the mistakes in division are understood, we might be able to prevent the abnormal proliferation of cells in tumors.