Four-Dimensional Heterogeneity of Fluid Phase Biopsies in Cancer (4DB-Center)

通过血流的肿瘤转移可以被建模为一个流固相互作用问题 在复杂的流场中运动的具有不同质量、动量和物理性质的物体(细胞)。 四维活检中心(4DB)利用基于物理的测量、数学建模 和数值模拟，以经验地表征实际的人类癌症患者样本，目标是 建立一种高度可预测的人类癌细胞行为模型，这将从根本上改变我们目前的 对癌症转移机制的理解。广泛的经验测量和分析 将对来自人类癌症患者的一组连贯的样本进行测试。样本将由细胞组成 从原发肿瘤，局部区域转移，和循环肿瘤细胞(CTC)随时间推移，所有收集 来自同一个病人。此外，第二批样本将由来自一大套 患者，按肿瘤类型和癌症分期分层。这些样本集将由三个人并行分析 独立研究项目(RPS)，产生相同现象的正交测量，以及 提供上皮性癌症在空间和时间维度上的变量和相关性。这个 三个研究项目(RP)是：RP1细胞物理学，它决定了物理和机械 癌细胞的特性；RP2拓扑学，它测量拓扑学、形态和传播组 肿瘤细胞的策略；以及RP3动态组学，它表征了肿瘤细胞的基因组学和转录组学 癌细胞。有了深度、广度和保真度的评估，测量将是 通过分析其他参数，包括标准的病理和临床患者信息。 这种广泛的数据融合，主要基于物理调查和数学理论，但 根据战略性的患者抽样和临床信息，将由高级统计处理 分析和数学建模，以实现对癌症扩散的高度预测性模拟 彻底改变了我们对转移的理解。4DB中心还将用于传播信息， 教育和培训新一代癌症物理学家；这一代人将实施这一力量 来攻克癌症的难题。

Cancer metastasis via the blood stream can be modeled as a problem of fluid-solid interaction with multiple bodies (cells) of different masses, momentum, and physical qualities, in motion, within a complex flow field. The Four Dimensional Biopsy Center (4DB) utilizes physics-based measurements, mathematical modeling and numerical simulation to empirically characterize actual human cancer patient samples, with a goal of establishing a highly predictive model of human cancer cell behavior that will fundamentally alter our current understanding of the mechanisms of cancer metastasis. Extensive empirical measurements and analyses will be performed on a coherent set of samples from human cancer patients. Samples will consist of cells from primary tumors, locoregional metastases, and circulating tumor cells (CTCs) over time, all collected from the same patient. Additionally, a second cohort of samples will consist of CTCs from a large set of patients, stratified by tumor type and cancer stage. These sample sets will be analyzed in parallel by three independent Research Projects (RPs), producing orthogonal measures of identical phenomena, and delivering the variables and the correlations on the space and time dimension of epithelial cancers. The three Research Projects (RP) are: RP1 Cytophysics, which determines the physical and mechanical properties of cancer cells; RP2 Topology, which measures the topology, morphology, and travel group strategies of cancer cells; and RP3 Dynomics, which characterizes the genomics and transcriptomics of cancer cells. With assessment of depth, breadth, and fidelity thus assured, the measurements will be empowerd by analyses of other parameters, including standard pathologic and clinical patient information. This extensive amalgamation of data, based primarily on physics investigations and mathematical theory, but informed by strategic patient sampling and clinical information, will be processed by advanced statistical analysis and mathematical modeling, to enable a highly predictive simulation of cancer spread that will revolutionize our understanding of metastasis. The 4DB Center will also serve to disseminate information, education, and training to a new generation of cancer physicists; a generation that will implement the power of physics to conquer the problems of cancer.