Nutrition and Physical Activity Assessment Study (NPAAS)

描述(由申请人提供)：R01CA119171的竞争续订，“营养和体力活动评估研究”(NPAAS)，继续关注使用生物标记物来评估和比较主要的营养和体力活动评估方法的测量特性，以及在妇女健康倡议(WHI)队列研究中使用生物标记物校准的营养消耗和与活动相关的能量消耗(Aree)估计。我们的目标是：1)进行受控喂养研究，以开发额外营养素和食物的生物标记物，以及2)使用正在进行的和拟议的NPAAS研究阶段的生物标记物，以开发整个WHI队列中用于疾病相关性研究的生物标记物校准的营养消耗和ARE估计。我们将招募150名女性，年龄范围在60-80岁之间，在西雅图赛场中心注册，进行高度个性化控制饮食的喂养研究，每个女性的饮食设计为接近她通常的食物消费模式。在两周的喂食期内，妇女将得到所有的食物和饮料。在两周喂食期开始和结束时，我们将获得24小时尿源性营养措施，包括氮(蛋白质)、果糖、蔗糖(糖)、烷基间苯二酚(全谷物)和1-和3-甲基组氨酸(肉类)；以及血源性营养措施，包括类胡萝卜素(水果和蔬菜)、1-和3-生育酚(脂肪和油)、磷脂脂肪酸(脂肪和油)以及叶酸(水果和蔬菜)。生物标记物评估将包括评估尿液和血液测量的能力，以及研究对象的特征，以解释喂养研究期间提供的营养和食物的变化。总能量消耗(TEE)将使用双标记水方案来测量，静息能量消耗(REE)将使用以0.9TEE-REE为客观衡量标准的间接量热法来测量。我们将通过使用来自NPAAS项目两个阶段的妇女的样本和数据，对相应的自我报告测量中的生物标记进行回归分析，为具有适当生物标记的每个营养/食物或活动衡量标准建立校准方程。合适的生物标记物将可用于总能量、蛋白质、来自蛋白质的能量百分比以及与活动相关的总能量消耗。我们将在WHI队列中应用这些校准方程，使用食物频率问卷和多天食物记录来产生校准的消费量估计，并使用WHI活动问卷来产生校准的ARE估计。这些经过校准的估计将与WHI队列中161,808名女性随后的一系列临床结果(例如癌症、心血管疾病、糖尿病、虚弱、肥胖等)的发病率有关。这项工作解决了饮食和体力活动流行病学研究、国家和国际肥胖症流行以及与此密切相关的慢性病发病率和死亡率方面的根本差距。

DESCRIPTION (provided by applicant): This competing renewal of R01 CA119171, "Nutrition and Physical Activity Assessment Study" (NPAAS), continues to focus on the use of biomarkers to evaluate and compare measurement properties of prominent nutritional and physical activity assessment methods, and on the use of biomarker-calibrated nutrient consumption and activity-related energy expenditure (AREE) estimates in studies in Women's Health Initiative (WHI) cohorts of postmenopausal women. Our aims are: 1) To conduct a controlled feeding study to develop biomarkers for additional nutrients and foods, and 2) To use biomarkers from the ongoing and proposed NPAAS study phases to develop biomarker-calibrated nutrient consumption and AREE estimates, throughout the WHI cohorts for use in disease-association studies. We will recruit 150 women, in the age range 60-80 y, enrolled in the WHI Seattle Field Center, into a feeding study of a highly individualized controlled diet, with each woman's diet designed to approximate her usual food consumption patterns. Women will receive all foods and beverages during the 2-week feeding period. We will obtain 24-h urine-derived nutritional measures, including nitrogen (protein), fructose, sucrose (sugars), alkylresorcinols (whole grains) and 1- and 3- methylhistidine (meat); and blood-derived nutritional measures, including carotenoids (fruits & vegetables), 1- and 3 -tocopherols (fats & oils), phospholipid fatty acids (fats & oils), and folate (fruits & vegetables) at the beginning and end of the 2-week feeding period. Biomarker evaluation will include an assessment of the ability of urine and blood measures, and study subject characteristics to explain variation in nutrients and foods provided during the feeding study. Total energy expenditure (TEE) will be measured using a doubly-labeled water protocol, as will resting energy expenditure (REE) using indirect calorimetry with 0.9TEE-REE as objective measure of AREE. We will develop calibration equations for each nutrient/food or activity measure having a suitable biomarker, by regression analysis of the biomarker on corresponding self-report measures using specimens and data from the women in both NPAAS project phases. Suitable biomarkers will be available for total energy, protein, percent of energy from protein, and for total activity-related energy expenditure. We will apply these calibration equations in the WHI cohorts using a food-frequency questionnaire and multiple-day food record to produce calibrated consumption estimates and using a WHI activity questionnaire to produce calibrated AREE estimates. These calibrated estimates will be related to the subsequent incidence of a broad range of clinical outcomes (e.g., cancer, cardiovascular disease, diabetes, frailty, obesity, etc) among the 161,808 women in the WHI cohorts. This work addresses fundamental gaps in diet and physical activity epidemiology research, in the context of national and international obesity epidemics, and in much related chronic disease morbidity and mortality.