Core F Regional Islet Production

摘要/概要 核心F：区域胰岛生产核心。与北方附近机构研究人员的互动 加州和太平洋西北部将加强整个地区的糖尿病研究。启动 互利的互动将通过扩大高度独特的糖尿病的可用性来加速， 中心设施内的UCSF刚果民主共和国我们的区域同事。这项工作将在即将提供的资金中启动。 通过向整个地区的研究人员开放UCSF世界级的胰岛生产核心，实现循环。通过提供 从小鼠和人类的胰腺中分离出的胰岛， 生产核心将使区域调查人员有机会将他们的发现转化为人类。 目的：区域胰岛生产核心巩固，增强和传播专业知识， 为目前正在研究或计划研究胰岛的DRC研究者提供胰岛制备方面的资源 细胞生物学、胰岛功能和/或移植。Core提供以下功能： 1.胰岛纯化。用于胰岛生物学的人和小鼠胰岛的加工和制备， 自身免疫和移植研究。 2.胰岛功能分析。胰岛素生产和其他的真实的实时生物和生化分析 人和小鼠胰岛的功能方面，以表征不同条件下的胰岛活性， 评估用于移植研究的胰岛制备质量。 对刚果民主共和国社区的好处：UCSF刚果民主共和国的胰岛生产核心一直收到来自 特别是在一段时间内，通过该地区的人类岛屿。核心的回应是建立了 确保获得研究级胰腺的采购和分配程序。它也有 建立了同样具有挑战性的协议，以确保实验室准备好接收 珍贵的人类胰岛通过在UCSF DRC内试行这些程序，胰岛生产 Core现在能够将这种能力扩展到整个地区的研究人员。五名区域调查员， 斯坦福大学、俄勒冈州健康科学大学、华盛顿大学表明需要人 我们每年准备的八种人类胰岛制剂中的每一种的研究胰岛。这24份 人类胰岛将通过将我们的胰岛制备从每年4个（用于UCSF）扩大到15个来适应 每年直接资助4，664，620美元的项目（8个，NIH资助3，501，837美元）将从这一努力中受益。 每年胰岛制备的数量增加一倍，以满足区域和UCSF DRC胰岛的需求 使研究人员有机会获得胰岛的频率加倍。这增强了对所有 将加速糖尿病研究，并使以前无法实现的研究使用人类胰岛。区域 研究人员还将获得由核心从模式生物分离的胰岛。因此，区域 核心将使整个地区的基础和翻译岛屿为基础的研究人员。

Abstract/Summary Core F: Regional Islet Production Core. Interactions with researchers at nearby institutions in Northern California and the Pacific Northwest would strengthen diabetes research throughout the region. The initiation of mutually beneficial interactions will be accelerated by extending the availability of highly unique, diabetes- centric facilities within the UCSF DRC to our regional colleagues. This will be initiated in the coming funding cycle by opening UCSF's world-class Islet Production Core to researchers throughout the region. By providing islets isolated from pancreases of mice and humans to researchers beyond UCSF, the Regional Islet Production Core will enable regional investigators the opportunity to translate their findings to humans. Purpose: The Regional Islet Production Core consolidates, enhances and disseminates expertise and resources in islet preparation to DRC investigators who currently study or have plans to study pancreatic islet cell biology, islet function and/or transplantation. The Core provides the following capabilities: 1. Islet Purification. Processing and preparation of human and mouse islets for use in islet biology, autoimmunity and transplantation studies. 2. Islet Functional Analysis. Real time biological and biochemical analysis of insulin production and other functional aspects of human and mouse islets to characterize islet activity under different conditions and to assess islet preparation quality for transplantation studies. Benefits to DRC Community: The UCSF DRC's Islet Production Core has been receiving requests from through the region for human islets, in particular, for some time. The Core has responded by establishing the procurement and distribution procedures that ensure access to research-grade pancreases. It also has established the equally challenging protocols for ensuring that laboratories are ready to receive the very valuable human islets, once prepared. By piloting these procedures within the UCSF DRC, the Islet Production Core is now able to extend this capability to researchers throughout the region. Five regional investigators from Stanford University, Oregon Health Sciences University, University of Washington indicate a need for human research islets for each of the eight human islet preparations we will prepare annually. These 24 aliquots of human islets will be accommodated by expanding our islet preparations from four per year (for UCSF) to 15 projects with $4,664,620 annual direct support (8, NIH-funded for $3,501,837) will benefit from this effort. Doubling the number of annual islet preparations to accommodate both regional and UCSF DRC islet needs doubles the frequency at which researchers have the opportunity to obtain islets. This enhanced access to all will accelerate diabetes research and enable previously unattainable studies using human islets. Regional investigators also will have access to islets isolated from model organisms by the Core. Thus, the Regional Core will enable both basic and translational islet-based researcher throughout the region.