Upgrade of a FACS Aria Cell Sorter

 描述（由申请人提供）：申请资金用于在佛蒙特大学流式细胞术和细胞分选（FCCS）设施安装相干50 mW 561 nm固态激光器并升级FACS Aria高速细胞分选仪。FCCS核心设施在医学院的房子目前在大学唯一的细胞分选。因此，这台仪器必须能够满足大学所有需要细胞分选的研究人员的需求。目前，该工具支持15个NIH支持的研究人员和27个NIH赠款，并为来自不同学科的广泛研究人员社区服务。研究重点的转移和新教师的招募都导致了许多研究人员需要对表达红色荧光蛋白的细胞进行分类，这些细胞最适合由561 nm激光线激发。由于我们目前的仪器不具备这种激光器，这些研究人员无法进行NIH资助的研究。我们已经确定，我们可以通过升级我们目前的仪器来满足研究人员的需求，以便安装561 nm激光器。这种升级只是购买新仪器费用的一小部分。升级后的仪器将继续位于已存在20多年的FCCS核心。该设施由经验丰富的认可科学家/细胞计量师管理，由具有20多年流式细胞术经验的教师顾问指导，并由内部咨询委员会监督。此外，该设施继续享有佛蒙特大学医学院的强有力的机构支持。

 DESCRIPTION (provided by applicant): A request is made for funds to install a Coherent 50 mW 561 nm solid-state laser and upgrade a FACS Aria high-speed cell sorter at the Flow Cytometry and Cell Sorting (FCCS) facility at the University of Vermont. The FCCS Core facility in the College of Medicine houses the only cell sorter present at the University. Therefore, this single instrument must be able to serve the needs of all investigators at the University who require cell sorting for their research. Currently, this instrument supports 15 NIH-supported investigators and 27 NIH grants and is serving a broad community of investigators from a variety of disciplines. Both shifting emphases in research and the recruitment of new faculty have resulted in a number of investigators who need to sort cells expressing red fluorescent proteins, which are optimally excited by a 561 nm laser line. Since our current instrument does not possess this laser, these investigators cannot conduct their NIH-funded research. We have determined that we can meet the needs of our investigators by upgrading our current instrument so that a 561 nm laser can be installed. This upgrade is a fraction of the cost of the purchase of a new instrument. The upgraded instrument will remain located in the FCCS core, which has been in existence for over 20 years. This facility is managed by an experienced and accredited scientist/cytometrist, is directed by a faculty advisor with over 20 years of experience in flow cytometry, and is overseen by an internal advisory committee. In addition, the facility continues to enjoy strong institutional support from the University Of Vermont College Of Medicine.