ChIP Sequencing Core

项目摘要(见说明)：GhlP-Seq生产核心将负责1)执行ChlP-分析；2)为llLumina下一代测序生成文库，3)将序列数据映射到KSHV和人类基因组，以及4)提供后续生物信息学分析。这些目标将是。与Wistar Institutes基因组和生物信息学核心合作完成，后者提供Lighina下一代测序作为核心服务。每个项目调查员都可以通过KSHV的共享网络浏览器访问最终数据管理和分析。芯片、下一代测序和生物信息学支持的所有方面的专业知识将在赠款支持的有效期内提供给项目。GhlP-Seq核心将由Paul Lieberman博士领导，他熟悉应用于KSHV和EBV基因组的芯片序列方法和生物信息学分析。ChlP-Seq核心将促进项目所有成员标准化样品制备和序列后分析方法的能力。这项工作将在利伯曼实验室内的维斯塔尔太空研究所进行，并将与维斯塔尔研究所的基因组核心和测序设施一起进行。

PROJECT SUMMARY (See instructions): The GhlP-Seq Production core will be responsible for 1) performing ChlP-assays; 2) generating library for lllumina next-generation sequencing, 3) mapping sequence data to KSHV and human genomes, and 4) providing follow-up bioinformatics analyses. These goals will be. accomplished in conjunction with the Wistar Institutes Genomics and Bioinformatics Core, which provides lllumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a shared web-based browser for KSHV. Expertise in all aspects of ChIP, Next-Generation Sequencing, and Bioinformatic support will be provided to the projects for the lifetime of grant support. The GhlP-Seq core will be lead by Dr. Paul Lieberman, who is familler with ChIP Seq methods and bioinformatics analyses as applied to KSHV and EBV genomes. The ChlP-Seq core will facilitate the ability of all members of the Program project to standardize, methods for sample preparation and for post-sequence analysis. The work will be performed at the Wistar Institute in space within the Lieberman lab; and in conjunction with the Wistar Institute Genomics Core and Sequencing facility.