Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development

描述（由申请人提供）：我们计划探索高度成功的减毒黄热病（YF-17D）疫苗，rDNA与IL-12和rAd35一起通过电穿孔传递，是否可以作为载体，在初始/增强方案中表达SIV蛋白，用于预防艾滋病病毒接种。在一系列高度整合的实验中，我们将首先确定疫苗的最佳病毒靶点。然后，我们将把结果汇总到最后的功效实验中，以测试病媒，如果成功，可以防止数百万人遭受痛苦

DESCRIPTION (provided by applicant): We plan to explore whether the highly successful attenuated yellow fever (YF-17D) vaccine, rDNA delivered by electroporation along with IL-12 and rAd35 can be used as vectors to express SIV proteins in a prime/boost regimen for vaccination against the AIDS virus. In a highly integrated series of experiments, we will first define the best viral targets for a vaccine. We will then bring the results together in a final efficacy experiment to test the vectors that, if successful, could prevent suffering by millions of people worldwide. Our application seeks to address four of the ten research areas outlined in the HIVRAD program announcement. Our project has the potential to impact HIV vaccine development on two fronts. First, we will define the targets of a successful vaccine in a challenge experiment. This will allow us to design a vaccine encoding the critical targets of a successful immune response. Second, we will develop three entirely novel vaccine vectors. If we are successful in this endeavor, our results will have a profound effect on HIV vaccine design.