Structured Illumination Fluorescence Microscope

 描述(由申请者提供)：购买蔡司Elyra S1超分辨率荧光显微镜需要资金。蔡司Elyra S1是一种3D结构化照明显微镜系统，可以对亚细胞结构进行最先进的成像。与传统的共聚焦和去卷积显微镜相比，该系统已经发展到了前所未有的程度，我们可以用多种颜色来分辨多个标记的结构。这项技术将以前最高250 nm的分辨率提高了一倍，并允许研究人员以三维方式定义位于相邻隔间的蛋白质之间的关系。这将大大提高我们对组织、细胞器和高阶蛋白质复合体的结构的了解。该仪器将支持6个NIH资助的主要用户和5个次要用户。虽然大多数用户在图森的亚利桑那大学，但该仪器将作为地区性资源提供。蔡司Elyra S1收集的数据将用于增加我们对心脏和骨骼肌功能和功能障碍、心血管和免疫功能、人类感染、导致癌症的细胞变化和基础细胞生物学的了解。能够在这种水平上分解细胞将使这些研究人员能够以意想不到和令人惊叹的方式推进他们的研究计划。

 DESCRIPTION (provided by applicant): Funds are requested to purchase a Zeiss Elyra S1 Super-Resolution fluorescent microscope. The Zeiss Elyra S1 is a 3D Structured Illumination Microscopy system that enables state-of-the-art imaging of subcellular structures. In contrast to conventional confocal and deconvolution microscopy, this system has advanced to such a degree that we can resolve multiple marked structures in multi-colors as never before. This technology doubles the previous maximum 250nm resolution and allows researchers to define the relationships between proteins that reside in adjacent compartments in three dimensions. This will substantially enhance our understanding of the structure of tissues, cellular organelles and higher order protein complexes. The instrument will support 6 NIH-funded major users and 5 minor users. While the majority of users are at The University of Arizona, Tucson, the instrument will be available as a regional resource. Data collected by the Zeiss Elyra S1 will be used to increase our understanding of heart and skeletal muscle function and dysfunction, cardiovascular and immune function, human infections, cellular alterations that lead to cancer and basic cell biology. Being able to resolve cells at this level will permit these investigators t push their research programs forward in unexpected and amazing ways.