JEOL JEM-1400 Transmission Electron Microscope

JEOL JEM-1400 透射电子显微镜

• 批准号: 8246819
• 负责人: David A Elliott
• 金额: $ 36.5万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246819
• 关键词: 21 year old; Adult; Aging; Arizona; Cardiovascular Physiology; Clinical; Colon Carcinoma; Data; Development; Electron Microscope; Functional disorder; Funding; Funding Applicant; Future; Health Sciences; Image; Letters; Location; Malignant neoplasm of esophagus; Microscope; Muscle function; Organ; Research; Research Personnel; Technology; Tissues; United States National Institutes of Health; Universities; digital imaging; heart function; immune function; instrument; meetings; repaired; toxicant; transmission process

DESCRIPTION (provided by applicant): Funds are requested in this proposal to purchase a JEOL JEM-1400 transmission electron microscope (TEM) to replace our aging, 21 year old Philips CM12S located in the Arizona Health Sciences Core Imaging Facility. The existing CM12S has increasing downtime for repairs with replacement parts becoming more difficult to obtain (FEI will only support the existing CM-12S for 30-36 months - see attached letter). The JEOL JEM-1400 will be the primary TEM available to basic and clinical researchers at the University of Arizona. Replacement of the aging microscope with a new TEM will insure that this technology is available to researchers at the University of Arizona for the foreseeable future. The JEOL JEM-1400 is a basic 120 kV TEM that meets the needs of our researchers. It is easy to use, has several digital imaging options and can be operated from remote locations. The instrument will support 8 NIH-funded major users who require the TEM for their research. Cellular and tissue ultra structural data collected using the JEOL JEM-1400 will be used to increase our understanding of colon and esophageal cancer, heart and muscle function and dysfunction, effects of toxicants during organ development, cardiovascular function, and immune function.

描述(由申请人提供)：本建议书要求资金购买JEOL JEM-1400透射电子显微镜(TEM)，以取代我们位于亚利桑那州健康科学核心成像设施的老化的、已有21年历史的飞利浦CM12S。现有的CM12S的维修停机时间越来越长，更换部件变得越来越难获得(FEI将仅支持现有的CM-12 30-36个月-见附信)。JEOL JEM-1400将是亚利桑那大学基础和临床研究人员可用的主要透射电子显微镜。用新的透射电子显微镜取代老化的显微镜将确保在可预见的未来亚利桑那大学的研究人员可以使用这项技术。JEOL JEM-1400是一种基本的120千伏瞬变电磁，满足了我们研究人员的需求。它使用方便，具有多个数字成像选项，并且可以从远程位置进行操作。该仪器将支持NIH资助的8个主要用户，他们的研究需要瞬变电磁。使用JEOL JEM-1400收集的细胞和组织超微结构数据将用于增加我们对结肠癌和食道癌、心脏和肌肉功能和功能障碍、有毒物质在器官发育过程中的影响、心血管功能和免疫功能的了解。

项目成果

The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation.

• DOI: 10.1038/s41467-022-28811-w
• 发表时间: 2022-03-11
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Khandelwal NK;Millan CR;Zangari SI;Avila S;Williams D;Thaker TM;Tomasiak TM
• 通讯作者: Tomasiak TM

A Conserved Motif in Intracellular Loop 1 Stabilizes the Outward-Facing Conformation of TmrAB.

细胞内环1中的保守基序稳定了TMRAB的向外构象。

• DOI: 10.1016/j.jmb.2021.166834
• 发表时间: 2021-08-06
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Millan CR;Francis M;Khandelwal NK;Thompson VF;Thaker TM;Tomasiak TM
• 通讯作者: Tomasiak TM

Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter.

• DOI: 10.1038/s41589-021-00936-x
• 发表时间: 2022-03
• 期刊: NATURE CHEMICAL BIOLOGY
• 影响因子: 14.8
• 作者: Thaker, Tarjani M.;Mishra, Smriti;Zhou, Wenchang;Mohan, Michael;Tang, Qingyu;Faraldo-Gomez, Jose D.;Mchaourab, Hassane S.;Tomasiak, Thomas M.
• 通讯作者: Tomasiak, Thomas M.

Cryo-EM structure of the tetra-phosphorylated R-domain in Ycf1 reveals key interactions for transport regulation.

Ycf1 中四磷酸化 R 结构域的冷冻电镜结构揭示了运输调节的关键相互作用。

• DOI: 10.1101/2024.03.06.583773
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: deCarvalho,RodolphoSA;Rasel,ShamiulI;Khandelwal,NiteshK;Tomasiak,ThomasM
• 通讯作者: Tomasiak,ThomasM