Flow Cytometry

流式细胞术是癌症研究的重要工具。它用于鉴定、跟踪和纯化特定的细胞 评估细胞周期进程和细胞凋亡反应，并支持广泛的 工程方法，如输送系统的量化和新蛋白质的开发 标签和成像技术。科赫研究所流式细胞术核心是一个共享资源， 为中心成员提供关于最先进的仪器、数据分析和 在细胞分类和分析方面的技术专长。在本期间，这一核心的能力进一步 进行了扩展和增强。这包括搬进新的科赫研究所一个更大的、定制设计的空间 建设、增加核心工作人员和购置新仪器。值得注意的是，在同一时期， 使用流式细胞核心的中心成员数量大幅增加 从78%到94%，包括所有四个项目的调查人员。因此，这种共享资源对于 科赫研究所任务的成功。在接下来的一段时间里，Flow细胞仪核心将继续 提供各种最先进的服务，以支持中心成员的研究计划。 此外，核心计划在麻省理工学院基金的支持下购买新的仪器，并建立 尖端单细胞技术，在所要求的发展资金的支持下。尽管我的专业是 服务扩展和由此导致的运行此核心的成本增加，要求CCSG为 与第三十九年所要求和建议的预算相比，第四十四年略有减少。

Flow cytometry is a critical tool for cancer research. It is used to identify, track and purify specific cell populations, assess cell cycle progression and apoptosis responses, and to support a broad range of engineering approaches such as the quantification of delivery systems and the development of novel protein labeling and imaging technologies. The Koch Institute Flow Cytometry Core is a Shared Resource that provides Center Members training in, and access to, state-of-the-art instrumentation, data analysis and technical expertise in cell sorting and analyses. In the current period, the capabilities of this Core were further expanded and enhanced. This includes moving into a larger, custom-designed space in the new Koch Institute building, an increase in the Core staff and the acquisition of new instrumentation. Notably, in the same period, there has been a substantial increase in the number of Center Members who use the Flow Cytomtery Core from 78% to 94%, including investigators from all four Programs. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, the Flow Cytometry Core will continue to offer a wide range of state-of-the-art services to support the research programs of Center Members. Additionally, the Core plans to make new instrument purchases, with the support of MIT funds, and establish cutting edge single cell technologies, with the support of requested developmental funds. In spite of the major expansion in services and the resulting increased costs of running this Core, the requested CCSG budget for Year 44 is slightly reduced from the requested and recommended budget in Year 39.