Cell and Molecular Analysis Core - "Mentoring in Cardiovascular Biology

今天，转化研究的进展需要分子、细胞、动物和生物学的复杂整合。 人体模型这种方法的多样性超出了“标准”实验室的可能性， 需要一个专门的环境，以提高成功率和增加我们的竞争力， investigators.细胞和分子分析核心是在第二年开始开发的阶段 I COBRE计划。今天，这个核心为我们的研究人员提供了LSUHSC上的独特设施 校园，它提供了最新的国家的最先进的细胞和分子生物学方法。 该核心提供熟练的人员来协助和/或执行高度专业化的实验 程序.该核心由Catalin Filipeanu博士和亚瑟哈斯博士协调， 以下单位：蛋白质组学，质谱，电子顺磁共振（EPR）成像，HPLC， 细胞培养和流式细胞术。该核心的具体目标是：1）提供技术人员协助 具有和/或执行用于蛋白质的HPLC分离、蛋白质的2D分离的高度专业化程序 用于通过质谱、细胞培养和EPR成像进行蛋白质组学分析，2）维持用于 安全有效的使用3）提供方法和仪器的教育和培训计划 在核心中可用，和4）协助调查人员在项目数据的生成。这些设施是开放的 所有COBRE研究人员，以及整个LSUHSC和其他机构的研究人员。的 多种类型的先进设备的可用性，复杂的数据分析系统，以及专业的 人员大大增加了当前和以前的COBRE和LSUHSC的研究成果 investigators.该核心的COBRE第三阶段过渡建立在我们之前的投资基础上， 大学致力于NCRR第一阶段和第二阶段计划。在第二阶段，本组织提供的服务 COBRE细胞和分子分析核心整合在LSUHSC蛋白质组学的保护伞下 因此，为将这一成功的核心设施转变为机构设施奠定了基础。

Today, the advance in translational research requires complex integration of molecular, cellular, animal and human models. This multitude of approaches exceeds the possibilities of a 'standard' laboratory and demands a specialized environment to enhance the success rate and increase the competiveness of our investigators. The Cell and Molecular Analysis Core was developed starting in the second year ofthe Phase I COBRE program. Today this core offers our researchers access to a unique facility on the LSUHSC campus, which provides the latest state-of the-art methodological approaches in cell and molecular biology. This core provides skilled personnel to assist with and/or perform highly specialized experimental procedures. This core is coordinated by Drs. Catalin Filipeanu and Arthur Haas and is organized in the following units: Proteomics, Mass Spectrometry, Electron Paramagnetic Resonance (EPR) Imaging, HPLC, Cell Culture, and Flow-cytometry. The Specific Aims of this core are: 1) to provide skilled personnel to assist with and/or perform highly specialized procedures for HPLC separation of proteins, 2D separation of proteins for proteomic analysis by mass spectrometry, cell culture, and EPR imaging, 2) to maintain the resources for safe and effective use 3) to provide programs of education and training ofthe methods and instrumentation available in the Core, and 4) to assist investigators in the generation of project data. These facilities are open to all COBRE investigators, as well as researchers throughout the LSUHSC and other institutions. The availability of multiple types of advanced equipment, sophisticated data analysis systems, and specialized personnel dramatically increase the research achievements by current and previous COBRE and LSUHSC investigators. The COBRE Phase III transition for this Core builds upon the previous investments that our University committed to the NCRR Phase I and Phase II programs. In Phase II, the services provided by this COBRE Cell and Molecular Analysis core were integrated under the umbrella ofthe LSUHSC Proteomics Facility, thus providing the foundation for transformation of this successful core to an Institutional facility.