Core C: Imaging and Cell Structure Core

肝脏中心的成像和细胞结构核心(ICSC)为肝脏中心的研究人员提供了 试剂、设备、分析工具和专业知识，以执行最先进的显微镜技术。这个 国际公务员制度委员会与我们的机构范围的分析成像设施(AIF)一起运作，后者提供 接触一流的荧光显微镜和电子显微镜并对其进行培训。进阶 核心中提供的分析成像技术在各个实验室中并不容易获得。这个 CORE通过支持和协助使用成像技术促进肝脏研究 专业仪器，包括激光扫描共聚焦显微镜、去卷积显微镜、多光子 显微镜和低温电子显微镜。此外，该中心还提供专业知识和援助。 具有专门的成像技术，如相关显微镜、囊泡跟踪、体积测量 测量、超微结构样品制备、光漂白后荧光恢复(FRAP)以及 荧光共振能量转移(FRET)。协助或独立使用此类设备的技术支持 提供仪器，肝脏中心(LC)调查人员获得优先和减价(10%) 使用设施仪器和服务。此外，还可为计划实验提供帮助 利用成像技术，解释光学和电子显微镜数据，设计荧光蛋白质融合， 并选择合适的荧光染料和蛋白质。酷睿拥有广泛的荧光产品目录 蛋白质质粒、染料标记抗体、细胞器标记物和其他可用于LC的试剂 调查人员。与Gruss Lipper生物光子学中心合作，CORE提供建议 更新设备，预见新出现的成像和细胞结构方法，并保持领先地位 为LC研究人员提供成像技术的前沿。

The Imaging and Cell Structure Core (ICSC) of the Liver Center provides Liver Center Investigators with the reagents, equipment, analytic tools, and expertise to perform 'state of the art' microscopy techniques. The ICSC operates in conjunction with our institution-wide Analytical Imaging Facility (AIF), which provides access to and training on maintained top ofthe line fluorescence and electron microscopes. Advanced analytical imaging techniques, available in the Core, are not readily available in individual laboratories. The Core facilitates use of imaging techniques in liver research by supporting and assisting with use of specialized instrumentation, including laser scanning confocal microscopy, deconvolution microscopy, multi-photon microscopy, and cryo-electron microscopy. In addition, the Core provides expertise in and assistance with specialized imaging techniques such as correlative microscopy, vesicle tracking, volumetric measurements, ultrastructural sample preparation, fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET). Technical support for assisted or independent use of such instrumentation is provided and Liver Center (LC) Investigators receive priority and reduced rates (10%) for use of facility instrumentation and services. In addition, assistance is available for planning experiments to utilize imaging techniques, interpret light and electron microscopic data, design fluorescent protein fusions, and select appropriate fluorescent dyes and proteins. The Core has an extensive catalog of fluorescent protein plasmids, dye-labeled antibodies, organelle markers, and other reagents available to LC Investigators. In conjunction with the Gruss Lipper Biophotonics Center, the Core provides recommendations to update equipment, anticipate emerging imaging and cell structure methods, and to remain at the leading edge of imaging technology for LC Investigators.